Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis
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Date
Authors
Yocgo, Rosita Endah
Creissen, Gary
Kunert, Karl J.
Chikwamba, R.K. (Rachel Kerina)
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Differences between the coding sequences of two banana homologues of the Non-expressor of Pathogenesis-Related 1 (NPR1) genes (MNPR1A and MNPR1B) were investigated as a possible cause for the differential activity of the two genes. Each of the MNPR1 coding sequences were expressed under the control of the cauliflower mosaic virus 35S promoter/terminator sequences in the transgenic Arabidopsis npr1-2 mutant. These MNPR1-expressing plants were then exposed to either the biotrophic oomycete Hyaloperonospora arabidopsidis, the necrotrophic fungus Botrytis cinerea, or the hemi-biotrophic bacterial pathogen Pseudomonas syringae. Expression of either MNPR1A or MNPR1B increased Pathogenesis-related 1 (PR-1) transcription in a similar manner in mutant plants and reduced pathogen growth, restoring resistance of the Arabidopsis npr1-2 mutant plants to pathogens. Sequence differences between MNPR1A and MNPR1B coding sequences did not affect MNPR1activity, suggesting a possible role for the involvement of other regulatory sequences in differential MNPR1 gene expression.
Description
Keywords
Banana NPR1-like sequences, Botrytis cinerea, Hyaloperonospora arabidopsidis, PR proteins, Pseudomonas syringae, Sequence dissimilarity, Non-expressor of Pathogenesis-Related 1 (NPR1)
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Citation
Yocgo, R.E., Creissen, G., Kunert, K.J. & Chikwamba, R.K. Two different banana NPR1-like coding sequences confer similar protection against pathogens in Arabidopsis. Tropical Plant Biology. (2012) 5: 309. doi:10.1007/s12042-012-9112-y.