Mitochondrial DNA parameters in blood of infants receiving lopinavir/ritonavir or lamivudine prophylaxis to prevent breastfeeding transmission of HIV-1

dc.contributor.authorMonnin, Audrey
dc.contributor.authorNagot, Nicolas
dc.contributor.authorPeries, Marianne
dc.contributor.authorVallo, Roselyne
dc.contributor.authorMeda, Nicolas
dc.contributor.authorSingata-Madliki, Mandisa
dc.contributor.authorTumwine, James K.
dc.contributor.authorKankasa, Chipepo
dc.contributor.authorNgandu, Nobubelo Kwanele
dc.contributor.authorGoga, Ameena Ebrahim
dc.contributor.authorReynier, Pascal
dc.contributor.authorTylleskar, Thorkild
dc.contributor.authorVan de Perre, Philippe
dc.contributor.authorMoles, Jean-Pierre
dc.date.accessioned2021-08-17T07:16:35Z
dc.date.available2021-08-17T07:16:35Z
dc.date.issued2020-09
dc.description.abstractChildren who are human immunodeficiency virus (HIV)-exposed but uninfected (CHEU) accumulate maternal HIV and antiretroviral exposures through pregnancy, postnatal prophylaxis, and breastfeeding. Here, we compared the dynamics of mitochondrial DNA (mtDNA) parameters in African breastfed CHEU receiving lopinavir/ritonavir (LPV/r) or lamivudine (3TC) pre-exposure prophylaxis during the first year of life. The number of mtDNA copies per cell (MCN) and the proportion of deleted mtDNA (MDD) were assessed at day 7 and at week 50 post-delivery (PrEP group). mtDNA depletion was defined as a 50% or more decrease from the initial value, and mtDNA deletions was the detection of mtDNA molecules with large DNA fragment loss. We also performed a sub-analysis with CHEU who did not receive a prophylactic treatment in South Africa (control group). From day seven to week 50, MCN decreased with a median of 41.7% (interquartile range, IQR: 12.1; 64.4) in the PrEP group. The proportion of children with mtDNA depletion was not significantly different between the two prophylactic regimens. Poisson regressions showed that LPV/r and 3TC were associated with mtDNA depletion (reference: control group; LPV/r: PR = 1.75 (CI95%: 1.15–2.68), p < 0.01; 3TC: PR = 1.54 (CI95%: 1.00–2.37), p = 0.05). Moreover, the proportion of children with MDD was unexpectedly high before randomisation in both groups. Long-term health impacts of these mitochondrial DNA parameters should be investigated further for both CHEU and HIV-infected children receiving LPV/r- or 3TC- based regimens.en_ZA
dc.description.departmentPaediatrics and Child Healthen_ZA
dc.description.librarianpm2021en_ZA
dc.description.urihttp://www.mdpi.com/journal/jcmen_ZA
dc.identifier.citationMonnin A, Nagot N, Periès M, Vallo R, Meda N, Singata-Madliki M, Tumwine JK, Kankasa C, Ngandu N, Goga A, Reynier P, Tylleskär T, Van de Perre P, Molès JP. Mitochondrial DNA Parameters in Blood of Infants Receiving Lopinavir/Ritonavir or Lamivudine Prophylaxis to Prevent Breastfeeding Transmission of HIV-1. Journal of Clinical Medicine 2020 Sep 14;9(9):2972. doi: 10.3390/jcm9092972. PMID: 32937988; PMCID: PMC7564660.en_ZA
dc.identifier.issn2077-0383 (online)
dc.identifier.other10.3390/jcm9092972
dc.identifier.urihttp://hdl.handle.net/2263/81305
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights© 2020 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_ZA
dc.subjectDeletionen_ZA
dc.subjectBreastfeedingen_ZA
dc.subjectAfricaen_ZA
dc.subjectHuman immunodeficiency virus (HIV)en_ZA
dc.subjectMitochondrial DNA (mtDNA)en_ZA
dc.subjectHIV-exposed uninfected children (CHEU)en_ZA
dc.subjectLopinavir/ritonavir (LPV/r)en_ZA
dc.subjectLamivudine (3TC)en_ZA
dc.titleMitochondrial DNA parameters in blood of infants receiving lopinavir/ritonavir or lamivudine prophylaxis to prevent breastfeeding transmission of HIV-1en_ZA
dc.typeArticleen_ZA

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