Nitric oxide inhibitory activity of Strychnos spinosa (Loganiaceae) leaf extracts and fractions

dc.contributor.authorIsa, Adamu Imam
dc.contributor.authorDzoyem, Jean Paul
dc.contributor.authorAdebayo, Salmon A.
dc.contributor.authorSuleiman, M.M.
dc.contributor.authorEloff, Jacobus Nicolaas
dc.contributor.emailkobus.eloff@up.ac.zaen_ZA
dc.date.accessioned2017-03-13T07:24:43Z
dc.date.available2017-03-13T07:24:43Z
dc.date.issued2016
dc.description.abstractBACKGROUND : The study was aimed at determining the anti-inflammatory activity of fractions and extracts obtained from Strychnos spinosa leaves on a mediator of inflammation nitric oxide (NO). MATERIALS AND METHODS : Leaves were extracted with acetone and separated into fractions with different polarities by solventsolvent fractionation. The Griess assay was used to determine the nitric oxide (NO) inhibitory activity. Cellular toxicity was determined by "using the MTT reduction assay". RESULTS : With the exception of the ethyl acetate fraction which had an IC50 >750 μg/mL, all extracts and fractions had significant nitric oxide-inhibitory activity. The most active being the water fraction, chloroform fraction and the dichloromethane/methanol extracts with IC50 values of 88.43 μg/mL, 96.72 μg/mL and 115.62 μg/mL, respectively. The extracts and fractions had low cytotoxicity on macrophage U937 cell lines. CONCLUSION : Extracts and fractions of Strychnos spinosa leaves may be promising sources of natural anti-inflammatory agents. Findings obtained from this study showed that Strychnos spinosa leaves possess promising anti-inflammatory action and could be used in the treatment of inflammation-related conditions.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe South Africa National Research Foundation (Grant IPPR 95991) and the University of Pretoria.en_ZA
dc.description.urihttp://journals.sfu.ca/africanem/index.php/ajtcamen_ZA
dc.identifier.citationIsa AI, Dzoyem, JP, Adebayo SA, Suleiman, MM & Eloff JN 2016, 'Nitric oxide inhibitory activity of Strychnos spinosa (Loganiaceae) leaf extracts and fractions', African Journal of Traditional, Complementary and Alternative Medicines, vol. 13, no. 6, pp. 22-26.en_ZA
dc.identifier.issn0189-6016
dc.identifier.other10.21010/ajtcam. v13i6.5
dc.identifier.urihttp://hdl.handle.net/2263/59393
dc.language.isoenen_ZA
dc.publisherCentre for Education Rights and Transformationen_ZA
dc.rightsThis article is licensed under the Creative Commons Attribution 3.0 Unported License.en_ZA
dc.subjectInflammationen_ZA
dc.subjectCytotoxicityen_ZA
dc.subjectNitric oxide (NO)en_ZA
dc.subjectStrychnos spinosa (Loganiaceae)en_ZA
dc.subjectStrychnos spinosa leavesen_ZA
dc.titleNitric oxide inhibitory activity of Strychnos spinosa (Loganiaceae) leaf extracts and fractionsen_ZA
dc.typeArticleen_ZA

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