Nitric oxide inhibitory activity of Strychnos spinosa (Loganiaceae) leaf extracts and fractions
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Date
Authors
Isa, Adamu Imam
Dzoyem, Jean Paul
Adebayo, Salmon A.
Suleiman, M.M.
Eloff, Jacobus Nicolaas
Journal Title
Journal ISSN
Volume Title
Publisher
Centre for Education Rights and Transformation
Abstract
BACKGROUND : The study was aimed at determining the anti-inflammatory activity of fractions and extracts obtained from Strychnos
spinosa leaves on a mediator of inflammation nitric oxide (NO).
MATERIALS AND METHODS : Leaves were extracted with acetone and separated into fractions with different polarities by solventsolvent
fractionation. The Griess assay was used to determine the nitric oxide (NO) inhibitory activity. Cellular toxicity was
determined by "using the MTT reduction assay".
RESULTS : With the exception of the ethyl acetate fraction which had an IC50 >750 μg/mL, all extracts and fractions had significant
nitric oxide-inhibitory activity. The most active being the water fraction, chloroform fraction and the dichloromethane/methanol
extracts with IC50 values of 88.43 μg/mL, 96.72 μg/mL and 115.62 μg/mL, respectively. The extracts and fractions had low
cytotoxicity on macrophage U937 cell lines.
CONCLUSION : Extracts and fractions of Strychnos spinosa leaves may be promising sources of natural anti-inflammatory agents.
Findings obtained from this study showed that Strychnos spinosa leaves possess promising anti-inflammatory action and could be
used in the treatment of inflammation-related conditions.
Description
Keywords
Inflammation, Cytotoxicity, Nitric oxide (NO), Strychnos spinosa (Loganiaceae), Strychnos spinosa leaves
Sustainable Development Goals
Citation
Isa AI, Dzoyem, JP, Adebayo SA, Suleiman, MM & Eloff JN 2016, 'Nitric oxide inhibitory activity of Strychnos spinosa (Loganiaceae) leaf extracts and fractions', African Journal of Traditional, Complementary and Alternative Medicines, vol. 13, no. 6, pp. 22-26.