Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks
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Date
Authors
Ambrosio, R.E.
Visser, Elizabeth S.
Koekhoven, Yolanda
Kocan, Katherine M.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Government Printer, Pretoria.
Abstract
DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization pattern suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Ambrosio, RE, Visser, ES, Koekhoven, Y & Kocan, KM 1988, 'Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks', Onderstepoort Journal of Veterinary Research, vol. 55, no. 4, pp. 227-229.