Staphylococcus aureus isolates from bovine mastitis in eight countries : genotypes, detection of genes encoding different toxins and other virulence genes

dc.contributor.authorMonistero, Valentina
dc.contributor.authorGraber, Hans Ulrich
dc.contributor.authorPollera, Claudia
dc.contributor.authorCremonesi, Paola
dc.contributor.authorCastiglioni, Bianca
dc.contributor.authorBottini, Enriqueta
dc.contributor.authorCeballos-Marquez, Alejandro
dc.contributor.authorLasso-Rojas, Laura
dc.contributor.authorKroemker, Volker
dc.contributor.authorWente, Nicole
dc.contributor.authorPetzer, Inge-Marie
dc.contributor.authorSantisteban, Carlos
dc.contributor.authorRunyan, Jeff
dc.contributor.authorVeiga dos Santos, Marcos
dc.contributor.authorAlves, Bruna Gomes
dc.contributor.authorPiccinini, Renata
dc.contributor.authorBronzo, Valerio
dc.contributor.authorAbbassi, Mohamed Salah
dc.contributor.authorBen Said, Meriam
dc.contributor.authorMoroni, Paolo
dc.contributor.emailinge-marie.petzer@up.ac.zaen_ZA
dc.date.accessioned2018-09-04T07:00:31Z
dc.date.available2018-09-04T07:00:31Z
dc.date.issued2018-06-17
dc.descriptionSupplementary File 1 : Unweighted pair-group method with arithmetic averages (UPGMA)-based dendrogram derived from the combined RS-PCR profiles and the virulence factors of the S. aureus strains considered in this study.en_ZA
dc.description.abstractStaphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.en_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.librarianam2018en_ZA
dc.description.urihttp://www.mdpi.com/journal/toxinsen_ZA
dc.identifier.citationMonistero, V., Graber, H.U., Pollera, C. et al. 2018, 'Staphylococcus aureus isolates from bovine mastitis in eight countries : genotypes, detection of genes encoding different toxins and other virulence genes', Toxins, vol. 10, art. no. 247, pp. 1-22.en_ZA
dc.identifier.issn2072-6651 (online)
dc.identifier.other10.3390/toxins10060247
dc.identifier.urihttp://hdl.handle.net/2263/66442
dc.language.isoenen_ZA
dc.publisherMDPI Publishingen_ZA
dc.rights2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_ZA
dc.subjectMastitisen_ZA
dc.subjectDairy cowen_ZA
dc.subjectGenotypesen_ZA
dc.subjectVirulence genesen_ZA
dc.subjectStaphylococcus aureusen_ZA
dc.subjectMultiplex polymerase chain reaction (M-PCR)en_ZA
dc.subjectWithin-herd prevalenceen_ZA
dc.subjectEpidemiologyen_ZA
dc.subjectIdentificationen_ZA
dc.subjectEnterotoxin genesen_ZA
dc.subjectIntramammary infections (IMI)en_ZA
dc.subjectSubclinical mastitisen_ZA
dc.subjectBulk tank milken_ZA
dc.subject.otherVeterinary science articles SDG-01en_ZA
dc.subject.otherVeterinary science articles SDG-02en_ZA
dc.subject.otherSDG-01: No poverty
dc.subject.otherSDG-02: Zero hunger
dc.titleStaphylococcus aureus isolates from bovine mastitis in eight countries : genotypes, detection of genes encoding different toxins and other virulence genesen_ZA
dc.typeArticleen_ZA

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