Staphylococcus aureus isolates from bovine mastitis in eight countries : genotypes, detection of genes encoding different toxins and other virulence genes

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Authors

Monistero, Valentina
Graber, Hans Ulrich
Pollera, Claudia
Cremonesi, Paola
Castiglioni, Bianca
Bottini, Enriqueta
Ceballos-Marquez, Alejandro
Lasso-Rojas, Laura
Kroemker, Volker
Wente, Nicole

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Publisher

MDPI Publishing

Abstract

Staphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.

Description

Supplementary File 1 : Unweighted pair-group method with arithmetic averages (UPGMA)-based dendrogram derived from the combined RS-PCR profiles and the virulence factors of the S. aureus strains considered in this study.

Keywords

Mastitis, Dairy cow, Genotypes, Virulence genes, Staphylococcus aureus, Multiplex polymerase chain reaction (M-PCR), Within-herd prevalence, Epidemiology, Identification, Enterotoxin genes, Intramammary infections (IMI), Subclinical mastitis, Bulk tank milk

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Citation

Monistero, V., Graber, H.U., Pollera, C. et al. 2018, 'Staphylococcus aureus isolates from bovine mastitis in eight countries : genotypes, detection of genes encoding different toxins and other virulence genes', Toxins, vol. 10, art. no. 247, pp. 1-22.