Safety and immunogenicity of plant‑produced African horse sickness virus‑like particles in horses

dc.contributor.authorDennis, Susan J.
dc.contributor.authorO’Kennedy, Martha M.
dc.contributor.authorRutkowska, Daria
dc.contributor.authorTsekoa, Tsepo
dc.contributor.authorLourens, Carina W.
dc.contributor.authorHitzeroth, Inga I.
dc.contributor.authorMeyers, Ann E.
dc.contributor.authorRybicki, Edward P.
dc.date.accessioned2019-02-08T05:47:26Z
dc.date.available2019-02-08T05:47:26Z
dc.date.issued2018-10-11
dc.descriptionAdditional file 1. Virus neutralising antibody titers and VP7 iELISA scores of VLP-vaccinated and control horse sera. Pre- and postvaccination sera were assayed for neutralisation capability against AHSV 5, AHSV 8 and AHSV 4. Sheep serum from animals vaccinated with live AHS virus was used as a positive control. The pre- and post-vaccination iELISA S/P scores of serum sampled prior to vaccination and 41 days post-vaccination are shown in the right hand column. High positive control sera pooled from vaccinated horses inoculated with live attenuated vaccine (bottle 1) obtained from OBP, was used as a positive control.en_ZA
dc.description.abstractAfrican horse sickness (AHS) is caused by multiple serotypes of the dsRNA AHSV and is a major scourge of domestic equids in Africa. While there are well established commercial live attenuated vaccines produced in South Africa, risks associated with these have encouraged attempts to develop new and safer recombinant vaccines. Previously, we reported on the immunogenicity of a plant-produced AHS serotype 5 virus-like particle (VLP) vaccine, which stimulated high titres of AHS serotype 5-specific neutralizing antibodies in guinea pigs. Here, we report a similar response to the vaccine in horses. This is the first report demonstrating the safety and immunogenicity of plant-produced AHS VLPs in horses.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2019en_ZA
dc.description.sponsorshipThe Technology and Innovation Agency (Grant No. TAHIC13-00023-CC). SD was supported by funding from the Technology and Innovation Agency, the Poliomyelitis Research Foundation and the Council for Scientific and Industrial Research.en_ZA
dc.description.urihttp://www.veterinaryresearch.orgen_ZA
dc.identifier.citationDennis, S.J., O'Kennedy, M.M., Rutkowska, D. et al. 2018, 'Safety and immunogenicity of plant‑produced African horse sickness virus‑like particles in horses', Veterinary Research, vol. 49, art. 105, pp. 1-6.en_ZA
dc.identifier.issn0928-4249 (print)
dc.identifier.issn1297-9716 (online)
dc.identifier.other10.1186/s13567-018-0600-4
dc.identifier.urihttp://hdl.handle.net/2263/68427
dc.language.isoenen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectAfricaen_ZA
dc.subjectAfrican horse sickness (AHS)en_ZA
dc.subjectVirus-like particle (VLP)en_ZA
dc.subjectHorse (Equus caballus)en_ZA
dc.subjectSafetyen_ZA
dc.subjectImmunogenicityen_ZA
dc.subjectVaccinesen_ZA
dc.titleSafety and immunogenicity of plant‑produced African horse sickness virus‑like particles in horsesen_ZA
dc.typeArticleen_ZA

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