Safety and immunogenicity of plant‑produced African horse sickness virus‑like particles in horses
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Date
Authors
Dennis, Susan J.
O’Kennedy, Martha M.
Rutkowska, Daria
Tsekoa, Tsepo
Lourens, Carina W.
Hitzeroth, Inga I.
Meyers, Ann E.
Rybicki, Edward P.
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
African horse sickness (AHS) is caused by multiple serotypes of the dsRNA AHSV and is a major scourge of domestic
equids in Africa. While there are well established commercial live attenuated vaccines produced in South Africa, risks
associated with these have encouraged attempts to develop new and safer recombinant vaccines. Previously, we
reported on the immunogenicity of a plant-produced AHS serotype 5 virus-like particle (VLP) vaccine, which stimulated
high titres of AHS serotype 5-specific neutralizing antibodies in guinea pigs. Here, we report a similar response
to the vaccine in horses. This is the first report demonstrating the safety and immunogenicity of plant-produced AHS
VLPs in horses.
Description
Additional file 1. Virus neutralising antibody titers and VP7 iELISA
scores of VLP-vaccinated and control horse sera. Pre- and postvaccination
sera were assayed for neutralisation capability against AHSV 5,
AHSV 8 and AHSV 4. Sheep serum from animals vaccinated with live AHS
virus was used as a positive control. The pre- and post-vaccination iELISA
S/P scores of serum sampled prior to vaccination and 41 days post-vaccination
are shown in the right hand column. High positive control sera
pooled from vaccinated horses inoculated with live attenuated vaccine
(bottle 1) obtained from OBP, was used as a positive control.
Keywords
Africa, African horse sickness (AHS), Virus-like particle (VLP), Horse (Equus caballus), Safety, Immunogenicity, Vaccines
Sustainable Development Goals
Citation
Dennis, S.J., O'Kennedy, M.M., Rutkowska, D. et al. 2018, 'Safety and immunogenicity
of plant‑produced African horse sickness
virus‑like particles in horses', Veterinary Research, vol. 49, art. 105, pp. 1-6.