Plaque formation by African horsesickness virus and characterization of its RNA
| dc.contributor.editor | South Africa. Dept. of Agricultural Technical Services | |
| dc.contributor.editor | Tustin, R.C. | |
| dc.contributor.editor | De Lange, M. | |
| dc.contributor.editor | Reinecke, R.K. | |
| dc.contributor.editor | Walker, Jane B. | |
| dc.contributor.editor | De Kock, V.E. | |
| dc.contributor.upauthor | Oellermann, R.A. | |
| dc.date.accessioned | 2015-07-08T11:23:17Z | |
| dc.date.available | 2015-07-08T11:23:17Z | |
| dc.date.created | 2013 | |
| dc.date.issued | 1970 | |
| dc.description | The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus production was observed. Sucrose gradient sedimentation analysis of AHSV-RNA revealed at least six components. Eight components were resolved by polyacrylamide gel electrophoresis. The molecular weight of the components varied from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of 1.5 x 10⁷ daltons for 10 segments in the viral genome. The significance of the relationship between AHSV, bluetongue virus and reovirus is discussed. | en |
| dc.description.librarian | mn2015 | |
| dc.description.sponsorship | . | en |
| dc.identifier.citation | Oellermann, RA 1970, 'Plaque formation by African horsesickness virus and characterization of its RNA', Onderstepoort Journal of Veterinary Research, vol. 37, no.2 , pp. 137-144. | en |
| dc.identifier.uri | http://hdl.handle.net/2263/46332 | |
| dc.language.iso | en | en |
| dc.publisher | Pretoria : Government Printer | en |
| dc.rights | ©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | Veterinary reports | en |
| dc.subject | South Africa | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | en |
| dc.subject.lcsh | African horse sickness | en |
| dc.subject.lcsh | Horses -- Virus diseases | en |
| dc.subject.lcsh | Orbivirus infections in animals | en |
| dc.title | Plaque formation by African horsesickness virus and characterization of its RNA | en |
| dc.type | Article | en |