Plaque formation by African horsesickness virus and characterization of its RNA
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Pretoria : Government Printer
Abstract
A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus production was observed. Sucrose gradient sedimentation analysis of AHSV-RNA revealed at least six components. Eight components were resolved by polyacrylamide gel electrophoresis. The molecular weight of the components varied from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of 1.5 x 10⁷ daltons for 10 segments in the viral genome. The significance of the relationship between AHSV, bluetongue virus and reovirus is discussed.
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The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.
Keywords
Veterinary medicine, Veterinary reports, South Africa
Sustainable Development Goals
Citation
Oellermann, RA 1970, 'Plaque formation by African horsesickness virus and characterization of its RNA', Onderstepoort Journal of Veterinary Research, vol. 37, no.2 , pp. 137-144.