Specific plasma microRNAs are associated with CD4+ T-cell recovery during suppressive antiretroviral therapy for HIV-1
dc.contributor.author | Kroeze, Stefanie | |
dc.contributor.author | Kootstra, Neeltje | |
dc.contributor.author | Van Nuenen, Ad C. | |
dc.contributor.author | Rossouw, Theresa M. | |
dc.contributor.author | Kityo, Cissy M. | |
dc.contributor.author | Siwale, Margaret | |
dc.contributor.author | Akanmu, Sulaimon | |
dc.contributor.author | Mandaliya, Kishor | |
dc.contributor.author | De Jager, Marleen | |
dc.contributor.author | Ondoa, Pascale | |
dc.contributor.author | Wit, Ferdinand W. | |
dc.contributor.author | Reiss, Peter | |
dc.contributor.author | Rinke de Wit, Tobias F. | |
dc.contributor.author | Hamers, Raph L. | |
dc.date.accessioned | 2024-08-30T13:14:08Z | |
dc.date.available | 2024-08-30T13:14:08Z | |
dc.date.issued | 2024-05 | |
dc.description | SUPPORTING INFORMATION: FILE S1: SUPPLEMENTAL DIGITAL CONTENT | en_US |
dc.description.abstract | OBJECTIVE: This study investigated the association of plasma microRNAs before and during antiretroviral therapy (ART) with poor CD4þ T-cell recovery during the first year of ART. DESIGN: MicroRNAs were retrospectively measured in stored plasma samples from people with HIV (PWH) in sub-Saharan Africa who were enrolled in a longitudinal multicountry cohort and who had plasma viral-load less than 50 copies/ml after 12 months of ART. METHODS: First, the levels of 179 microRNAs were screened in a subset of participants from the lowest and highest tertiles of CD4þ T-cell recovery (DCD4) (N ¼ 12 each). Next, 11 discordant microRNAs, were validated in 113 participants (lowest tertile DCD4: n ¼ 61, highest tertile DCD4: n ¼ 52). For discordant microRNAs in the validation, a pathway analysis was conducted. Lastly, we compared microRNA levels of PWH to HIV-negative controls. RESULTS: Poor CD4þ T-cell recovery was associated with higher levels of hsa-miR-199a3p and hsa-miR-200c-3p before ART, and of hsa-miR-17-5p and hsa-miR-501-3p during ART. Signaling by VEGF and MET, and RNA polymerase II transcription pathways were identified as possible targets of hsa-miR-199a-3p, hsa-200c-3p, and hsamiR-17-5p. Compared with HIV-negative controls, we observed lower hsa-miR-326, hsa-miR-497-5p, and hsa-miR-501-3p levels before and during ART in all PWH, and higher hsa-miR-199a-3p and hsa-miR-200c-3p levels before ART in all PWH, and during ART in PWH with poor CD4þ T-cell recovery only. CONCLUSION: These findings add to the understanding of pathways involved in persistent HIV-induced immune dysregulation during suppressive ART. | en_US |
dc.description.department | Immunology | en_US |
dc.description.sdg | SDG-03:Good heatlh and well-being | en_US |
dc.description.sponsorship | A Veni postdoc fellowship to R.L.H. through the Dutch Research Council (NWO) Talent Programme (91615036), and Gilead Sciences Netherlands through an unrestricted scientific grant. | en_US |
dc.description.uri | https://journals.lww.com/aidsonline/pages/default.aspx | en_US |
dc.identifier.citation | Kroeze, S., Kootstra, N., Van Nuenen, A.C. et al. 2024, 'Specific plasma microRNAs are associated with CD4+ T-cell recovery during suppressive antiretroviral therapy for HIV-1', AIDS, vol. 38, pp. 791-801, doi : 10.1097/QAD.0000000000003853. | en_US |
dc.identifier.issn | 0269-9370 (print) | |
dc.identifier.issn | 1473-5571 (online) | |
dc.identifier.other | 10.1097/QAD.0000000000003853 | |
dc.identifier.uri | http://hdl.handle.net/2263/97965 | |
dc.language.iso | en | en_US |
dc.publisher | Lippincott, Williams & Wilkins | en_US |
dc.rights | © 2024 The Author(s). | en_US |
dc.subject | CD4þ T-cell recovery | en_US |
dc.subject | HIV-1 | en_US |
dc.subject | Immune dysregulation | en_US |
dc.subject | MicroRNA | en_US |
dc.subject | Antiretroviral therapy (ART) | en_US |
dc.subject | People with HIV (PWH) | en_US |
dc.subject | Human immunodeficiency virus (HIV) | en_US |
dc.subject | SDG-03: Good health and well-being | en_US |
dc.title | Specific plasma microRNAs are associated with CD4+ T-cell recovery during suppressive antiretroviral therapy for HIV-1 | en_US |
dc.type | Article | en_US |
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