Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
dc.contributor.author | Mosaka, Thabang B.M. | |
dc.contributor.author | Unuofin, John Onolame | |
dc.contributor.author | Daramola, Michael Olawale | |
dc.contributor.author | Tizaoui, Chedly | |
dc.contributor.author | Iwarere, Samuel Ayodele | |
dc.contributor.email | samuel.iwarere@up.ac.za | en_US |
dc.date.accessioned | 2024-08-27T09:05:28Z | |
dc.date.available | 2024-08-27T09:05:28Z | |
dc.date.issued | 2024-08 | |
dc.description | AVAILABILITY OF DATA AND MATERIALS : Sources of data collected have been mentioned in the text. | en_US |
dc.description.abstract | Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs. | en_US |
dc.description.department | Chemical Engineering | en_US |
dc.description.librarian | hj2024 | en_US |
dc.description.sdg | SDG-06:Clean water and sanitation | en_US |
dc.description.sdg | SDG-12:Responsible consumption and production | en_US |
dc.description.sponsorship | The Government of the United Kingdom through The Royal Society FLAIR award. Open access funding provided by University of Pretoria. | en_US |
dc.description.uri | https://www.springer.com/journal/11356 | en_US |
dc.identifier.citation | Mosaka, T.B.M., Unuofin, J.O., Daramola, M.O. et al. Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor. Environmental Science and Pollution Research 31, 49811–49822 (2024). https://doi.org/10.1007/s11356-024-34475-4. | en_US |
dc.identifier.issn | 0944-1344 (print) | |
dc.identifier.issn | 1614-7499 (online) | |
dc.identifier.other | 10.1007/s11356-024-34475-4 | |
dc.identifier.uri | http://hdl.handle.net/2263/97891 | |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.rights | © The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.subject | Wastewater treatment plant (WWTP) | en_US |
dc.subject | Antibiotic-resistant bacteria (ARB) | en_US |
dc.subject | Antibiotic-resistant genes (ARGs) | en_US |
dc.subject | Non-thermal plasma (NTP) | en_US |
dc.subject | Acinetobacter baumannii | en_US |
dc.subject | Carbapenem-resistant gene | en_US |
dc.subject | Cold atmospheric plasma | en_US |
dc.subject | Disinfection | en_US |
dc.subject | SDG-06: Clean water and sanitation | en_US |
dc.subject | SDG-12: Responsible consumption and production | en_US |
dc.title | Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor | en_US |
dc.type | Article | en_US |