Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor

dc.contributor.authorMosaka, Thabang B.M.
dc.contributor.authorUnuofin, John Onolame
dc.contributor.authorDaramola, Michael Olawale
dc.contributor.authorTizaoui, Chedly
dc.contributor.authorIwarere, Samuel Ayodele
dc.contributor.emailsamuel.iwarere@up.ac.zaen_US
dc.date.accessioned2024-08-27T09:05:28Z
dc.date.available2024-08-27T09:05:28Z
dc.date.issued2024-08
dc.descriptionAVAILABILITY OF DATA AND MATERIALS : Sources of data collected have been mentioned in the text.en_US
dc.description.abstractWastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs.en_US
dc.description.departmentChemical Engineeringen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-06:Clean water and sanitationen_US
dc.description.sdgSDG-12:Responsible consumption and productionen_US
dc.description.sponsorshipThe Government of the United Kingdom through The Royal Society FLAIR award. Open access funding provided by University of Pretoria.en_US
dc.description.urihttps://www.springer.com/journal/11356en_US
dc.identifier.citationMosaka, T.B.M., Unuofin, J.O., Daramola, M.O. et al. Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor. Environmental Science and Pollution Research 31, 49811–49822 (2024). https://doi.org/10.1007/s11356-024-34475-4.en_US
dc.identifier.issn0944-1344 (print)
dc.identifier.issn1614-7499 (online)
dc.identifier.other10.1007/s11356-024-34475-4
dc.identifier.urihttp://hdl.handle.net/2263/97891
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.rights© The Author(s) 2024. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectWastewater treatment plant (WWTP)en_US
dc.subjectAntibiotic-resistant bacteria (ARB)en_US
dc.subjectAntibiotic-resistant genes (ARGs)en_US
dc.subjectNon-thermal plasma (NTP)en_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectCarbapenem-resistant geneen_US
dc.subjectCold atmospheric plasmaen_US
dc.subjectDisinfectionen_US
dc.subjectSDG-06: Clean water and sanitationen_US
dc.subjectSDG-12: Responsible consumption and productionen_US
dc.titleNon-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactoren_US
dc.typeArticleen_US

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