Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
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Date
Authors
Mosaka, Thabang B.M.
Unuofin, John Onolame
Daramola, Michael Olawale
Tizaoui, Chedly
Iwarere, Samuel Ayodele
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs.
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AVAILABILITY OF DATA AND MATERIALS :
Sources of data collected have been mentioned in the text.
Keywords
Wastewater treatment plant (WWTP), Antibiotic-resistant bacteria (ARB), Antibiotic-resistant genes (ARGs), Non-thermal plasma (NTP), Acinetobacter baumannii, Carbapenem-resistant gene, Cold atmospheric plasma, Disinfection, SDG-06: Clean water and sanitation, SDG-12: Responsible consumption and production
Sustainable Development Goals
SDG-06:Clean water and sanitation
SDG-12:Responsible consumption and production
SDG-12:Responsible consumption and production
Citation
Mosaka, T.B.M., Unuofin, J.O., Daramola, M.O. et al. Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor. Environmental Science and Pollution Research 31, 49811–49822 (2024). https://doi.org/10.1007/s11356-024-34475-4.