Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR
| dc.contributor.author | Wang, Zheng-Wei | |
| dc.contributor.author | Vreede, F.T. | |
| dc.contributor.author | Mitchell, J.O. | |
| dc.contributor.author | Viljoen, G.J. | |
| dc.contributor.editor | Boomker, Jacob Diederik Frederik | |
| dc.date.accessioned | 2012-12-12T06:35:45Z | |
| dc.date.available | 2012-12-12T06:35:45Z | |
| dc.date.created | 2012 | |
| dc.date.issued | 2001 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en_US |
| dc.description.abstract | A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted NDV-infected allantoic fluid and found to be 10ˉ⁵ HA units. Application of one-step RT-PCR to various NDV samples, including wild-type virulent isolates and avirulent vaccine strains, demonstrated the potential for rapid identification (3-4 h) of NDV isolates as well as the differentiation of virulent from avirulent strains. | en_US |
| dc.identifier.citation | Wang, Z, Vreede, FT, Mitchell, JO & Viljoen, GJ 2001, 'Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 131-134. | en_US |
| dc.identifier.issn | 0030-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/20686 | |
| dc.language | en | |
| dc.publisher | Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. | en_US |
| dc.rights | © ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital). | en_US |
| dc.subject | Veterinary medicine | en_US |
| dc.subject | Detection | en_US |
| dc.subject | Identification | en_US |
| dc.subject | Newcastle disease virus (NDV) | en_US |
| dc.subject | RT-PCR | en_US |
| dc.subject | Triton X-100 differentiation of NDV isolates | en_US |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR | en_US |
| dc.type | Article | en_US |