Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia

dc.contributor.authorAdamcikova, Katarina
dc.contributor.authorJanosikova, Zuzana
dc.contributor.authorVan der Nest, Ariska
dc.contributor.authorAdamcik, Slavomir
dc.contributor.authorOndruskova, Emilia
dc.contributor.authorBarnes, Irene
dc.date.accessioned2022-07-19T05:01:39Z
dc.date.available2022-07-19T05:01:39Z
dc.date.issued2021-10
dc.description.abstractDothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.departmentPlant Production and Soil Scienceen_US
dc.description.librarianhj2022en_US
dc.description.sponsorshipVedecká Grantová Agentúra MŠVVaŠ SR a SAV and Tree Protection Co-operative Programme.en_US
dc.description.urihttp://www.wileyonlinelibrary.com/journal/ppaen_US
dc.identifier.citationAdamčíková K, Jánošíková Z, van der Nest A, Adamčík S, Ondrušková E, Barnes I. Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia. Plant Pathology 2021;70:1883– 1896. https://doi.org/10.1111/ppa.13428.en_US
dc.identifier.issn0032-0862 (print)
dc.identifier.issn1365-3059 (online)
dc.identifier.other10.1111/ppa.13428
dc.identifier.urihttps://repository.up.ac.za/handle/2263/86288
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2021 British Society for Plant Pathology. This is the pre-peer reviewed version of the following article : Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia. Plant Pathology 2021;70:1883– 1896. https://doi.org/10.1111/ppa.13428. The definite version is available at : http://www.wileyonlinelibrary.com/journal/ppa.en_US
dc.subjectAscomycete fungusen_US
dc.subjectClonalityen_US
dc.subjectMicrosatellitesen_US
dc.subjectMultiplex PCR (mPCR)en_US
dc.subjectSexual recombinationen_US
dc.titlePopulation structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakiaen_US
dc.typePostprint Articleen_US

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