Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia

Loading...
Thumbnail Image

Authors

Adamcikova, Katarina
Janosikova, Zuzana
Van der Nest, Ariska
Adamcik, Slavomir
Ondruskova, Emilia
Barnes, Irene

Journal Title

Journal ISSN

Volume Title

Publisher

Wiley

Abstract

Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.

Description

Keywords

Ascomycete fungus, Clonality, Microsatellites, Multiplex PCR (mPCR), Sexual recombination

Sustainable Development Goals

Citation

Adamčíková K, Jánošíková Z, van der Nest A, Adamčík S, Ondrušková E, Barnes I. Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia. Plant Pathology 2021;70:1883– 1896. https://doi.org/10.1111/ppa.13428.