Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory
| dc.contributor.author | Brauer, Marieke | |
| dc.contributor.author | Wolfaardt, Marianne | |
| dc.contributor.author | Webber, Lynne M. | |
| dc.contributor.author | Taylor, Maureen B. | |
| dc.contributor.email | marieke.brauer@up.ac.za | en_ZA |
| dc.date.accessioned | 2016-06-06T05:59:12Z | |
| dc.date.available | 2016-06-06T05:59:12Z | |
| dc.date.issued | 2016 | |
| dc.description | The information contained in this article was presented by Marieke Brauer at the UNIPATH 2014 conference, held on 19-21 September 2014 at the CSIR Convention Centre in Pretoria (no abstract number available). | en_ZA |
| dc.description.abstract | The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis. | en_ZA |
| dc.description.department | Medical Virology | en_ZA |
| dc.description.librarian | am2016 | en_ZA |
| dc.description.uri | http://www.sajei.co.za/index.php/SAJEI | en_ZA |
| dc.identifier.citation | Brauer, M, Marianne Wolfaardt, M, Webber, LM & Taylor, MB 2016, 'Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory', Southern African Journal of Infectious Diseases, vol. 31, no. 1, pp. 29-31. | en_ZA |
| dc.identifier.issn | 2312-0053 (print) | |
| dc.identifier.issn | 2313-1810 (online) | |
| dc.identifier.other | 10.1080/23120053.2016.1118843 | |
| dc.identifier.uri | http://hdl.handle.net/2263/52868 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Medpharm Publications, NISC (Pty) Ltd and Cogent, Taylor & Francis Group | en_ZA |
| dc.rights | © 2016 The Author(s). Open Access article distributed under the terms of the Creative Commons License [CC BY-NC-ND 4.0] http://creativecommons.org/licenses/by-nc-nd/4.0. | en_ZA |
| dc.subject | Aseptic meningitis | en_ZA |
| dc.subject | Central nervous system | en_ZA |
| dc.subject | Genotype | en_ZA |
| dc.subject | Polymerase chain reaction | en_ZA |
| dc.subject | Mumps virus (MuV) | en_ZA |
| dc.subject | Cerebrospinal fluid (CSF) | en_ZA |
| dc.subject | Real-time reverse transcription-polymerase chain reaction (RT-PCR) | en_ZA |
| dc.title | Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory | en_ZA |
| dc.type | Article | en_ZA |