Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory
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Date
Authors
Brauer, Marieke
Wolfaardt, Marianne
Webber, Lynne M.
Taylor, Maureen B.
Journal Title
Journal ISSN
Volume Title
Publisher
Medpharm Publications, NISC (Pty) Ltd and Cogent, Taylor & Francis Group
Abstract
The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically
characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the
MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of
specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains.
As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated
with aseptic meningitis.
Description
The information contained in this article was
presented by Marieke Brauer at the UNIPATH 2014 conference, held on 19-21 September 2014 at the CSIR Convention Centre in
Pretoria (no abstract number available).
Keywords
Aseptic meningitis, Central nervous system, Genotype, Polymerase chain reaction, Mumps virus (MuV), Cerebrospinal fluid (CSF), Real-time reverse transcription-polymerase chain reaction (RT-PCR)
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Citation
Brauer, M, Marianne Wolfaardt, M, Webber, LM & Taylor, MB 2016, 'Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory', Southern African Journal of Infectious Diseases, vol. 31, no. 1, pp. 29-31.