Characterisation of oil degrading bacteria from tailored compost containing crude oil sludge

dc.contributor.authorUbani, Onyedikachi
dc.contributor.authorAtagana, Harrison Ifeanyichukwu
dc.contributor.authorThantsha, Mapitsi Silvester
dc.contributor.authorAdeleke, Rasheed
dc.date.accessioned2017-02-20T06:04:41Z
dc.date.available2017-02-20T06:04:41Z
dc.date.issued2016-04
dc.description.abstractIn the present study, different bacteria with polycyclic aromatic hydrocarbon (PAH)-degrading capabilities were isolated from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identification using polymerase chain reaction (PCR) of the 16S rRNA gene with specific primers (16S-P1 PCR (5¢AGAGTTTGATCCTGGCTCAG3¢) and 16S-P2 PCR (5¢AAGGAGGTGATCCAGCCGCA3¢). The amplicons were sequenced using 16S-PA SEQ (5/CTACGGGAGGCAGCAG3/) and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades: Firmicutes, Proteobacteria and Actinobacteria. The bacteria identified were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAH in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAH of different mol wt in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation.en_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe South African National Research Foundationen_ZA
dc.description.urihttp://www.sherpa.ac.uk/romeo/issn/0972-5849/en_ZA
dc.identifier.citationUbani, O, Atagana, AH, Thantsha, MS & Adeleke, R 2016, 'Characterisation of oil degrading bacteria from tailored compost containing crude oil sludge', Indian Journal of Biotechnology, vol. 15, pp. 243-250.en_ZA
dc.identifier.issn0972-5849
dc.identifier.urihttp://hdl.handle.net/2263/59102
dc.language.isoenen_ZA
dc.publisherNational Institute of Science Communication and Information Resourcesen_ZA
dc.rightsNational Institute of Science Communication and Information Resourcesen_ZA
dc.subjectAnimal manuresen_ZA
dc.subjectBioaugmentationen_ZA
dc.subjectBiodegradationen_ZA
dc.subjectBioremediationen_ZA
dc.subjectComposingen_ZA
dc.subjectOil sludgeen_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectPolycyclic aromatic hydrocarbon (PAH)en_ZA
dc.titleCharacterisation of oil degrading bacteria from tailored compost containing crude oil sludgeen_ZA
dc.typeArticleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Ubani_Characterisation_2016.pdf
Size:
236.75 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: