The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus
| dc.contributor.author | Du Plessis, D.H. | |
| dc.contributor.author | Van Wyngaardt, Wouter | |
| dc.contributor.author | Romito, M. | |
| dc.contributor.author | Du Plessis, M. | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.contributor.upauthor | Maree, Sarita | |
| dc.date.accessioned | 2012-10-01T11:48:59Z | |
| dc.date.available | 2012-10-01T11:48:59Z | |
| dc.date.created | 2012 | |
| dc.date.issued | 1999 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken lgY directed against AHSV (serotype 3) was used as the secondary antibody. Since lgY and mouse lgG do not cross-react serologically, the secondary antibody was not labelled, but was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins. The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses. | en |
| dc.description.librarian | mn2012 | en |
| dc.identifier.citation | Du Plessis, DH, Van Wyngaardt, W, Romito, M, Du Plessis, M & Maree, S 1999, 'The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 66, no. 1, pp. 25-28. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/19926 | |
| dc.language.iso | en | en |
| dc.publisher | Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute | en |
| dc.rights | ©ARC-Onderstepoort (original). ©University of Pretoria. Dept of Library Services (digital). | en |
| dc.subject | African horse sickness | en |
| dc.subject | Antibody sandwich | en |
| dc.subject | Chickens | en |
| dc.subject | Egg-yolk | en |
| dc.subject | Orbiviruses | en |
| dc.subject | AHS | |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.subject.lcsh | African horse sickness -- South Africa | en |
| dc.subject.lcsh | Veterinary virology | en |
| dc.subject.lcsh | Horses -- Diseases | en_ZA |
| dc.title | The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus | en |
| dc.type | Article | en |