The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus
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Date
Authors
Du Plessis, D.H.
Van Wyngaardt, Wouter
Romito, M.
Du Plessis, M.
Journal Title
Journal ISSN
Volume Title
Publisher
Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV)
was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody
specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk
derived chicken lgY directed against AHSV (serotype 3) was used as the secondary antibody. Since
lgY and mouse lgG do not cross-react serologically, the secondary antibody was not labelled, but
was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins.
The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis
virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole
AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses.
Description
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.
Keywords
African horse sickness, Antibody sandwich, Chickens, Egg-yolk, Orbiviruses, AHS
Sustainable Development Goals
Citation
Du Plessis, DH, Van Wyngaardt, W, Romito, M, Du Plessis, M & Maree, S 1999, 'The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 66, no. 1, pp. 25-28.