The partial purification of Clostridium perfringens Beta toxin
| dc.contributor.author | Mulders, M.S.G. | |
| dc.contributor.author | Worthington, R.W. | |
| dc.contributor.editor | Bigalke, R.D. | |
| dc.contributor.editor | Cameron, Colin McKenzie | |
| dc.contributor.editor | Verster, Anna J,M. | |
| dc.contributor.editor | Walker, Jane B. | |
| dc.contributor.other | De Kock, V.E. | |
| dc.date.accessioned | 2016-07-06T11:10:13Z | |
| dc.date.available | 2016-07-06T11:10:13Z | |
| dc.date.created | 2016 | |
| dc.date.issued | 1975 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acroabt XI was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en_ZA |
| dc.description.abstract | An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified, was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorption spectrum in the UV region, an iso-electric point of 5, 6 and the main component had a molecular mass of 42 000 ± 2 000 (estimated by electrophoresis in sodium dodecyl sulphate containing polyacrylamide gels) | en_ZA |
| dc.identifier.citation | Worthington, RW & Mulders, MSG 1975, 'The partial purification of Clostridium perfringens Beta toxin’, Onderstepoort Journal of Veterinary Research, vol. 42, no. 3, pp. 91-98. | en_ZA |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/53723 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | Published by The Government Printer, Pretoria | en_ZA |
| dc.rights | ©1975 ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria. Department of Library Services (digital). | en_ZA |
| dc.subject | Veterinary medicine | en_ZA |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | The partial purification of Clostridium perfringens Beta toxin | en_ZA |
| dc.type | Article | en_ZA |