The partial purification of Clostridium perfringens Beta toxin
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Date
Authors
Mulders, M.S.G.
Worthington, R.W.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by The Government Printer, Pretoria
Abstract
An attempt was made to purify Clostridium perfringens Beta toxin. Crude toxin prepared by ammonium sulphate precipitation of culture supernatants was purified by chromatography on Sephadex G50, Sephadex G100 and DEAE cellulose. This material, although highly purified, was not homogeneous on polyacrylamide gel electrophoresis. It had a toxicity of 800 000 mouse MLDs/mg N, a typical protein absorption spectrum in the UV region, an iso-electric point of 5, 6 and the main component had a molecular mass of 42 000 ± 2 000 (estimated by electrophoresis in sodium dodecyl sulphate containing polyacrylamide gels)
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Worthington, RW & Mulders, MSG 1975, 'The partial purification of Clostridium perfringens Beta toxin’, Onderstepoort Journal of Veterinary Research, vol. 42, no. 3, pp. 91-98.