Stability, morphology, and effects of in vitro digestion on the antioxidant properties of polyphenol inclusion complexes with -cyclodextrin

dc.contributor.authorNtuli, Sunday
dc.contributor.authorLeuschner, Machel
dc.contributor.authorBester, Megan
dc.contributor.authorSerem, June Cheptoo
dc.contributor.emailjune.serem@up.ac.zaen_US
dc.date.accessioned2023-09-21T10:02:22Z
dc.date.available2023-09-21T10:02:22Z
dc.date.issued2022-06-14
dc.descriptionDATA AVAILABILTY STATEMENT : Some data is available as supplementary material and the rest under the UPSpace institutional repository, URI: http://hdl.handle.net/2263/84453 (date dissertation was available online 11 March 2022).en_US
dc.descriptionSUPPLEMENTARY MATERIAL : FIGURE S1: Enhanced product ions scan mass spectrum of CD inclusion complexes with (a) CAT, (b) GA, and (c) EGCG. CD—beta cyclodextrin, CAT—catechin, GA—gallic acid, EGCG—epigallocatechin gallate; FIGURE S2: Inhibition of AGEs formation by non-digested (ND) and following simple (SD) and complex (CD) digestion of nonencapsulated and encapsulated CAT/GA, CAT/EGCG, and GA/EGCG combinations evaluated with the (a) BSA-MGO and (b) BSA-FRU models at 100 M for each polyphenol in each sample. The data is represented as the mean SEM of at least five experiments performed in duplicates. ND—nondigested, SD—simple digestion, CD—complex digestion, AGEs—advanced glycation end-products, BSA—bovine serum albumin, MGO—methylglyoxal, FRU—fructose, CAT—catechin, GA—gallic acid, EGCG—epigallocatechin gallate. The * and + represent significant (p < 0.05) differences between non-encapsulated and encapsulated ND compared with the respective SD or CD samples. The denotes significant (p < 0.05) differences between the non-encapsulated and encapsulated for each treatment; TABLE S1: Polyphenol–polyphenol interactions on antioxidant activity (ORAC assay ( M TE)); TABLE S2: Polyphenol–polyphenol interactions on antiglycation activity (AGEs assay (%)); TABLE S3: Polyphenol–polyphenol interactions on cellular antioxidant activity (DCFH-DA assay (%)).en_US
dc.description.abstractPolyphenols are inversely associated with the incidence of chronic diseases, but therapeutic use is limited by poor stability and bioaccessibility. Encapsulation has been shown to overcome some of these limitations. A selection of polyphenols (catechin, gallic acid, and epigallocatechin gallate) and their combinations were encapsulated in beta-cyclodextrin (CD). Encapsulation was characterized and the thermal and storage stability was evaluated using the 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) assay. The samples were then subjected to in vitro digestion using a simple digestion (SD) model (gastric and duodenal phases) and a more complex digestion (CD) model (oral, gastric, and duodenal phases). Thereafter, the chemical (oxygen radical absorbance capacity assay) and cellular (dichlorofluorescein diacetate assay in Caco-2 cells) antioxidant and antiglycation (advanced glycation end-products assay) activities were determined. Inclusion complexes formed at a 1:1 molar ratio with a high encapsulation yield and efficiency. Encapsulation altered the morphology of the samples, increased the thermal stability of some and the storage stability of all samples. Encapsulation maintained the antioxidant activity of all samples and significantly improved the antiglycation and cellular antioxidant activities of some polyphenols following SD. In conclusion, the formed inclusion complexes of CD with polyphenols had greater storage stability, without altering the beneficial cellular effects of the polyphenols.en_US
dc.description.departmentAnatomyen_US
dc.description.departmentPharmacologyen_US
dc.description.librarianam2023en_US
dc.description.sponsorshipThe National Research Foundation South Africa and the APC was funded by the University of Pretoria, Faculty of Health Sciences Library and the University of Pretoria, Faculty of Health Sciences.en_US
dc.description.urihttps://www.mdpi.com/journal/moleculesen_US
dc.identifier.citationNtuli, S.; Leuschner, M.; Bester, M.J.; Serem, J.C. Stability, Morphology, and Effects of In Vitro Digestion on the Antioxidant Properties of Polyphenol Inclusion Complexes with -Cyclodextrin. Molecules 2022, 27, 3808. https://DOI.org/10.3390/molecules27123808.en_US
dc.identifier.issn1420-3049 (online)
dc.identifier.other10.3390/molecules27123808
dc.identifier.urihttp://hdl.handle.net/2263/92374
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectPolyphenolsen_US
dc.subjectAntioxidanten_US
dc.subjectAntiglycationen_US
dc.subjectEncapsulationen_US
dc.subjectBeta-cyclodextrinen_US
dc.subject.otherHealth sciences articles SDG-03
dc.subject.otherSDG-03: Good health and well-being
dc.titleStability, morphology, and effects of in vitro digestion on the antioxidant properties of polyphenol inclusion complexes with -cyclodextrinen_US
dc.typeArticleen_US

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