RESTseq – efficient benchtop population genomics with RESTriction fragment SEQuencing
| dc.contributor.author | Stolle, Eckart | |
| dc.contributor.author | Moritz, Robin F.A. | |
| dc.contributor.editor | Wade, Claire | |
| dc.date.accessioned | 2013-07-12T07:23:14Z | |
| dc.date.available | 2013-07-12T07:23:14Z | |
| dc.date.issued | 2013-05-17 | |
| dc.description.abstract | We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by comparing two honeybee and several stingless bee samples. | en |
| dc.description.librarian | am2013 | en |
| dc.description.librarian | ab2013 | |
| dc.description.sponsorship | European Union’s Seventh Framework Programme (grant agreement BEE DOC FP7-KBBE-2009-3 244956 CP-FP), Bundesministerium fu¨ r Erna¨hrung, Landwirtschaft und Verbraucherschutz (project FITBEE). | en |
| dc.description.uri | http://www.plosone.org | en |
| dc.identifier.citation | Stolle E, Moritz RFA (2013) RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing. PLoS ONE 8(5): e63960. DOI: 10.1371/journal.pone.0063960 | en |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.other | 10.1371/journal.pone.0063960 | |
| dc.identifier.uri | http://hdl.handle.net/2263/21910 | |
| dc.language.iso | en | en |
| dc.publisher | Public Library of Science | en |
| dc.rights | © 2013 Stolle, Moritz. This is an open-access article distributed under the terms of the Creative Commons Attribution License | en |
| dc.subject | RESTseq | en |
| dc.subject | SNP detection | en |
| dc.subject | SNP genotyping | en |
| dc.subject | Next generation sequencing (NGS) | en |
| dc.subject.lcsh | Metagenomics | en |
| dc.subject.lcsh | DNA | en |
| dc.title | RESTseq – efficient benchtop population genomics with RESTriction fragment SEQuencing | en |
| dc.type | Article | en |