RESTseq – efficient benchtop population genomics with RESTriction fragment SEQuencing
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Date
Authors
Stolle, Eckart
Moritz, Robin F.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments
from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome
sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from
fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate
the validity of our approach by comparing two honeybee and several stingless bee samples.
Description
Keywords
RESTseq, SNP detection, SNP genotyping, Next generation sequencing (NGS)
Sustainable Development Goals
Citation
Stolle E, Moritz RFA (2013) RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing. PLoS ONE 8(5): e63960. DOI: 10.1371/journal.pone.0063960