Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues

dc.contributor.authorRagan, Izabela K.
dc.contributor.authorSchuck, Kaitlynn N.
dc.contributor.authorUpreti, Deepa
dc.contributor.authorOdendaal, Lieza
dc.contributor.authorRicht, Juergen A.
dc.contributor.authorTrujillo, Jessie D.
dc.contributor.authorWilson, William C.
dc.contributor.authorDavis, A.S. (Sally)
dc.contributor.emaillieza.odendaal@up.ac.zaen_ZA
dc.date.accessioned2019-07-30T08:44:03Z
dc.date.issued2019-06
dc.descriptionThis work was done in partial completion of Izabela Ragan’s PhD dissertation.en_ZA
dc.description.abstractSporadic outbreaks of Rift Valley fever virus (RVFV), a zoonotic, mosquito-borne Phlebovirus, cause abortion storms and death in sheep and cattle resulting in catastrophic economic impacts in endemic regions of Africa. More recently, with changes in competent vector distribution, growing international trade, and its potential use for bioterrorism, RVFV has become a transboundary animal disease of significant concern. New and sensitive techniques that determine RVFV presence, while lessening the potential for environmental contamination and human risk, through the use of inactivated, noninfectious samples such as formalin-fixed, paraffin-embedded (FFPE) tissues are needed. FFPE tissue in situ hybridization (ISH) enables the detection of nucleic acid sequences within the visual context of cellular and tissue morphology. Here, we present a chromogenic pan-RVFV ISH assay based on RNAscope® technology, which is able to detect multiple RVFV strains in FFPE tissues, enabling visual correlation of RVFV RNA presence with histopathologic lesions.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.embargo2020-06-26
dc.description.librarianhj2019en_ZA
dc.description.sponsorshipThe USDA Agricultural Research Service and the Department of Diagnostic Medicine, College of Veterinary Medicine, Kansas State University’s startup funds for Dr. Davis. This work was also supported through the Department of Homeland Security (grant no. 2010-ST061-AG0001), Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD).en_ZA
dc.description.urihttp://online.liebertpub.com/VBZen_ZA
dc.identifier.citationRagan, I.K., Schuck, K.N., Upreti, D. et al. 2019, 'Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues', Vector Borne and Zoonotic Diseases, vol. 19, no. 7, pp. 553-556.en_ZA
dc.identifier.issn1530-3667 (print)
dc.identifier.issn1557-7759 (online)
dc.identifier.other10.1089/vbz.2018.2383
dc.identifier.urihttp://hdl.handle.net/2263/70820
dc.language.isoenen_ZA
dc.publisherMary Ann Lieberten_ZA
dc.rights© 2019 Mary Ann Liebert, Inc. All rights reserved.en_ZA
dc.subjectRift Valley fever virus (RVFV)en_ZA
dc.subjectFormalin-fixed, paraffin-embedded (FFPE)en_ZA
dc.subjectIn situ hybridization (ISH)en_ZA
dc.titleRift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissuesen_ZA
dc.typePostprint Articleen_ZA

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