Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues
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Date
Authors
Ragan, Izabela K.
Schuck, Kaitlynn N.
Upreti, Deepa
Odendaal, Lieza
Richt, Juergen A.
Trujillo, Jessie D.
Wilson, William C.
Davis, A.S. (Sally)
Journal Title
Journal ISSN
Volume Title
Publisher
Mary Ann Liebert
Abstract
Sporadic outbreaks of Rift Valley fever virus (RVFV), a zoonotic, mosquito-borne Phlebovirus, cause abortion storms and death in sheep and cattle resulting in catastrophic economic impacts in endemic regions of Africa. More recently, with changes in competent vector distribution, growing international trade, and its potential use for bioterrorism, RVFV has become a transboundary animal disease of significant concern. New and sensitive techniques that determine RVFV presence, while lessening the potential for environmental contamination and human risk, through the use of inactivated, noninfectious samples such as formalin-fixed, paraffin-embedded (FFPE) tissues are needed. FFPE tissue in situ hybridization (ISH) enables the detection of nucleic acid sequences within the visual context of cellular and tissue morphology. Here, we present a chromogenic pan-RVFV ISH assay based on RNAscope® technology, which is able to detect multiple RVFV strains in FFPE tissues, enabling visual correlation of RVFV RNA presence with histopathologic lesions.
Description
This work was done in partial completion of Izabela Ragan’s PhD dissertation.
Keywords
Rift Valley fever virus (RVFV), Formalin-fixed, paraffin-embedded (FFPE), In situ hybridization (ISH)
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Citation
Ragan, I.K., Schuck, K.N., Upreti, D. et al. 2019, 'Rift Valley fever viral RNA detection by in situ hybridization in formalin-fixed, paraffin-embedded tissues', Vector Borne and Zoonotic Diseases, vol. 19, no. 7, pp. 553-556.