DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps

dc.contributor.authorDittrich-Schroder, Gudrun
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorKlein, Hildegard
dc.contributor.authorSlippers, Bernard
dc.contributor.emailbernard.slippers@fabi.up.ac.zaen_US
dc.date.accessioned2012-03-19T07:13:17Z
dc.date.available2013-01-31T10:07:45Z
dc.date.issued2012-01
dc.description.abstractDNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR of the cox 1 mitochondrial locus. A silicamembrane- based approach was the most successful, followed by a method using a combination of incubation buffers and amethod using magnetic beads. The method using buffers was the most cost- and time effective. Using this method, larvae from Eucalyptus seed capsule galls could be assigned a role (parasitoid, gall former or inquiline) in the gall-inhabiting complex.en
dc.description.librariannf2012en
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-0998en_US
dc.identifier.citationDittrich-Schroder, G, Wingfield, MJ, Klein, H & Slippers, B 2012, 'DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps', Molecular Ecology Resources, vol. 12, no. 1, pp. 1-19.en
dc.identifier.issn1755-098X (print)
dc.identifier.issn1755-0998 (online)
dc.identifier.other10.1111/j.1755-0998.2011.03074.x
dc.identifier.urihttp://hdl.handle.net/2263/18473
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.rights© 2011 Blackwell Publishing Ltd. The definite version is available at onlinelibrary.wiley.com. This article is embargoed by the publisher until January 2013.en
dc.subjectDNA extractionen
dc.subjectCox 1en
dc.subjectMinute insectsen
dc.subjectMtDNA barcodingen
dc.subject.lcshAnimal genetics -- Techniqueen
dc.subject.lcshHymenoptera -- Geneticsen
dc.subject.lcshWasps -- Geneticsen
dc.subject.lcshDNA fingerprinting of animalsen
dc.titleDNA extraction techniques for DNA barcoding of minute gall-inhabiting waspsen
dc.typePostprint Articleen

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