DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps
dc.contributor.author | Dittrich-Schroder, Gudrun | |
dc.contributor.author | Wingfield, Michael J. | |
dc.contributor.author | Klein, Hildegard | |
dc.contributor.author | Slippers, Bernard | |
dc.contributor.email | bernard.slippers@fabi.up.ac.za | en_US |
dc.date.accessioned | 2012-03-19T07:13:17Z | |
dc.date.available | 2013-01-31T10:07:45Z | |
dc.date.issued | 2012-01 | |
dc.description.abstract | DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR of the cox 1 mitochondrial locus. A silicamembrane- based approach was the most successful, followed by a method using a combination of incubation buffers and amethod using magnetic beads. The method using buffers was the most cost- and time effective. Using this method, larvae from Eucalyptus seed capsule galls could be assigned a role (parasitoid, gall former or inquiline) in the gall-inhabiting complex. | en |
dc.description.librarian | nf2012 | en |
dc.description.uri | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-0998 | en_US |
dc.identifier.citation | Dittrich-Schroder, G, Wingfield, MJ, Klein, H & Slippers, B 2012, 'DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps', Molecular Ecology Resources, vol. 12, no. 1, pp. 1-19. | en |
dc.identifier.issn | 1755-098X (print) | |
dc.identifier.issn | 1755-0998 (online) | |
dc.identifier.other | 10.1111/j.1755-0998.2011.03074.x | |
dc.identifier.uri | http://hdl.handle.net/2263/18473 | |
dc.language.iso | en | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.rights | © 2011 Blackwell Publishing Ltd. The definite version is available at onlinelibrary.wiley.com. This article is embargoed by the publisher until January 2013. | en |
dc.subject | DNA extraction | en |
dc.subject | Cox 1 | en |
dc.subject | Minute insects | en |
dc.subject | MtDNA barcoding | en |
dc.subject.lcsh | Animal genetics -- Technique | en |
dc.subject.lcsh | Hymenoptera -- Genetics | en |
dc.subject.lcsh | Wasps -- Genetics | en |
dc.subject.lcsh | DNA fingerprinting of animals | en |
dc.title | DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps | en |
dc.type | Postprint Article | en |
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