DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps

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Authors

Dittrich-Schroder, Gudrun
Wingfield, Michael J.
Klein, Hildegard
Slippers, Bernard

Journal Title

Journal ISSN

Volume Title

Publisher

Wiley-Blackwell

Abstract

DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of each method was scored on a 2% agarose gel after PCR of the cox 1 mitochondrial locus. A silicamembrane- based approach was the most successful, followed by a method using a combination of incubation buffers and amethod using magnetic beads. The method using buffers was the most cost- and time effective. Using this method, larvae from Eucalyptus seed capsule galls could be assigned a role (parasitoid, gall former or inquiline) in the gall-inhabiting complex.

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Keywords

DNA extraction, Cox 1, Minute insects, MtDNA barcoding

Sustainable Development Goals

Citation

Dittrich-Schroder, G, Wingfield, MJ, Klein, H & Slippers, B 2012, 'DNA extraction techniques for DNA barcoding of minute gall-inhabiting wasps', Molecular Ecology Resources, vol. 12, no. 1, pp. 1-19.