Freeze-drying of Aegyptianella pullorum
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Date
Authors
Bartkowiak, R.A.
Potgieter, F.T.
Van Rensburg, L.
Labuschagne, F.J.
Van Biljon, B.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Government Printer, Pretoria.
Abstract
Heparinized whole blood, parasitized with Aegyptianella pullorum, was collected from 2 fowls. Buffered lactose peptone (BLP) was added v/v as a stabilizer and the mixture lyophilized in 2ml aliquots after rapid or slow freezing. At different stages during the freeze-drying process, as well as after lyophilization and reconstitution with I ,8 ml of sterile water, samples were taken and injected into pullets. Infectivity was maintained throughout. However, the prepatent period was lengthened after freezing and particularly after lyophilization when there was some loss of viability.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Bartkowiak, RA, Huchzermeyer, FW, Potgieter, FT, Van Rensburg, L, Labuschagne, FJ & Van Biljon, BJ 1988, 'Freeze-drying of Aegyptianella pullorum', Onderstepoort Journal of Veterinary Research, vol. 55, no. 2, pp. 125-126.