An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera
| dc.contributor.author | Williamson, Catherine C. | |
| dc.contributor.author | Oberem, P.T. | |
| dc.contributor.author | Poerstamper, C. | |
| dc.contributor.author | De Waal, D.T. | |
| dc.contributor.author | Matthee, O. | |
| dc.contributor.author | Brett, O.L. | |
| dc.contributor.editor | Bigalke, R.D. | |
| dc.date.accessioned | 2014-10-07T10:29:29Z | |
| dc.date.available | 2014-10-07T10:29:29Z | |
| dc.date.created | 2014 | |
| dc.date.issued | 1988 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | An ELISA was developed using an SDS extract of Brucella abortus as antigen to detect antibodies in cattle sera. The antigen was stable at 4 °C for at least 4 years and although it gave optimal results at a 1/4 000 dilution it could detect reactors at a 1/32 000 dilution. Based on comparative CF tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. The ELISA could be a useful test to supplement the CF test but further evaluation is still required. | en |
| dc.description.librarian | lmchunu2014 | en |
| dc.description.librarian | mn2014 | |
| dc.identifier.citation | Williamson, CC, Oberem, PT, Poerstamper, C, De Waal, DT, Matthee, O, & Brett, OL 1988, 'An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera', Onderstepoort Journal of Veterinary Research, vol. 55, no. 1, pp. 1-3. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/42270 | |
| dc.language.iso | en | en |
| dc.publisher | Published by the Government Printer, Pretoria. | en |
| dc.rights | ©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera | en |
| dc.type | Article | en |