A chemically defined medium for the growth of Cowdria ruminantium
| dc.contributor.author | Josemans, A.I. | |
| dc.contributor.editor | Boomker, Jacob Diederik Frederik | |
| dc.contributor.upauthor | Zweygarth, Erich | |
| dc.date.accessioned | 2012-03-20T12:13:57Z | |
| dc.date.available | 2012-03-20T12:13:57Z | |
| dc.date.created | 2012 | |
| dc.date.issued | 2001 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | Chemically defined media, termed SFMC-23 and SFMC-36, were devised for the in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants. Both media were based on Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 (DME/F-12) containing various supplements. Medium SFMC-23 and SFMC-36 supported the long-term growth of the Welgevonden stock of C. ruminantium for a total of 55 and 28 passages, respectively, with regular passage intervals of 3 days. Using SFMC-23, split ratios varied from 5-10, depending on which host cell line was used. Other stocks of C. ruminantium (Sankat, Blaauwkrantz, Senegal) were successfully propagated for a test period of ten passages. | en |
| dc.description.librarian | mn2012 | en |
| dc.description.sponsorship | Agricultural Research Council of South Africa. European Union (Cowdriosis Network) Grant no. IC18-CT95-0008 (DG12-SNRD). | en |
| dc.identifier.citation | Zweygarth, E & Josemans, AI 2001, 'A chemically defined medium for the growth of Cowdria ruminantium’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 1, pp. 37-40. | en |
| dc.identifier.issn | 0030-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/18484 | |
| dc.language | en | |
| dc.publisher | Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. | en |
| dc.rights | © ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | Chemically defined medium | en |
| dc.subject | Cowdria ruminantium | en |
| dc.subject | In vitro culture | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.subject.lcsh | Heartwater -- South Africa | en |
| dc.subject.lcsh | Tick-borne diseases in animals | en |
| dc.title | A chemically defined medium for the growth of Cowdria ruminantium | en |
| dc.type | Article | en |