Root exudate compounds change the bacterial community in bulk soil

dc.contributor.authorAfzal, Muhammad Yasir
dc.contributor.authorDas, Bikram K.
dc.contributor.authorValappil, Vishnu Thayil
dc.contributor.authorScaria, Joy
dc.contributor.authorBrözel, Volker Siegfried
dc.date.accessioned2024-04-24T07:38:42Z
dc.date.issued2024-06
dc.descriptionDATA AVAILABILITY : Data will be made available on request.en_US
dc.description.abstractThe soil bacteria are diverse in nature both physiologically and phylogenetically with spatial variations within the soil microenvironments. Plant roots secrete organic substances called root exudates which benefit bacteria able to incorporate these. Subsequently, as the root grows, it changes the organic carbon status of adjacent bulk soil, stimulating growth of some of the resident bacteria. This growth induces a shift in the soil bacterial community and causes modifications in its metabolic activities. This nutrient infusion could also activate resting structures such as endospores to grow. We asked how the bulk soil microbial community responds when encountering root exudates and hypothesized that bacteria able to grow rapidly would become predominant upon introduction of root exudates. We added synthetic root exudate cocktail (Dietz et al., 2020) to the bulk soil from a wheat field on day 0 and day 1. We determined the aerobic culturable count on R2A, and Bacillus cereus sensu lato on Mannitol Egg Yolk Polymyxin agar, and bacterial community composition by sequencing the V3–V4 regions of the 16S rRNA genes on days 0, 1, 2, 3, 4, 6, 8, 10, 12 and 14 of incubation. Alpha diversity (Shannon) decreased and recovered partially, indicating a shift in species evenness while the Chao1 index remained the same, indicating constant species richness. Beta diversity shifted substantially over time. Rare fast-growing genera like Paenarthrobacter and Pseudarthrobacter increased upon REC addition, while slow growing genera like Bradyrhizobium were constant over time. Some key genera like Stenotrophobacter responded only after ceasing of REC addition. Certain fast-growing genera like Bacillus did not increase in population density. Collectively, these results indicate that the bulk soil community shifted significantly when exposed to REC, and after termination of REC, continued to undergo shifts. This presents the root environment with diverse bacteria known to benefit growth, such as Paenarthrobacter and rhizobia.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.embargo2025-04-09
dc.description.librarianhj2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipHigher Education Commission of Pakistan (HEC-Pakistan) for PhD scholarship program, the National Science Foundation, and by the South Dakota Board of Regents.en_US
dc.description.urihttps://www.elsevier.com/locate/rhisphen_US
dc.identifier.citationAfzal, M.Y., Das, B.K., Valappil, V.T. et al. 2024, 'Root exudate compounds change the bacterial community in bulk soil', Rhizosphere, vol. 30, art. 100885, pp. 1-11, doi : 10.1016/j.rhisph.2024.100885.en_US
dc.identifier.issn2452-2198 (online)
dc.identifier.other10.1016/j.rhisph.2024.100885
dc.identifier.urihttp://hdl.handle.net/2263/95740
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2024 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Rhizosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Rhizosphere, vol. 30, art. 100885, pp. 1-11, 2024, doi : 10.1016/j.rhisph.2024.100885.en_US
dc.subjectRhizosphereen_US
dc.subjectBacterial diversityen_US
dc.subjectRare biosphereen_US
dc.subjectRoot exudateen_US
dc.subjectConditionally rare taxaen_US
dc.subjectSDG-15: Life on landen_US
dc.titleRoot exudate compounds change the bacterial community in bulk soilen_US
dc.typePostprint Articleen_US

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