Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family

dc.contributor.authorAdesioye, Fiyinfoluwa Adenike
dc.contributor.authorMakhalanyane, Thulani Peter
dc.contributor.authorVikram, Surendra
dc.contributor.authorSewell, Bryan T.
dc.contributor.authorSchubert, Wolf-Dieter
dc.contributor.authorCowan, Don A.
dc.contributor.emaildon.cowan@up.ac.zaen_ZA
dc.date.accessioned2018-04-20T10:19:35Z
dc.date.issued2018-04
dc.description.abstractPlease read abstract in the article.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentGeneticsen_ZA
dc.description.embargo2018-10-01
dc.description.librarianhj2018en_ZA
dc.description.sponsorshipThe South Africa Bio-catalysis Initiative, Department of Science and Technology, the University of Pretoria Genomics Research Institute (D.A.C. and T.P.M.), the National Research Foundation (W.-D.S. and B.T.S.), the Research Development Program (T.P.M. and S.V.), and the Organization for Women in Science in the Developing World (OWSD) (F.A.A.).en_ZA
dc.description.urihttp://aem.asm.orgen_ZA
dc.identifier.citationAdesioye FA, Makhalanyane TP, Vikram S, Sewell BT, Schubert W-D, Cowan DA. 2018. Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family. Appl Environ Microbiol 84:e02695-17. https://doi.org/10.1128/AEM.02695-17.en_ZA
dc.identifier.issn0099-2240 (print)
dc.identifier.issn1098-5336 (online)
dc.identifier.other10.1128/AEM.02695-17
dc.identifier.urihttp://hdl.handle.net/2263/64669
dc.language.isoenen_ZA
dc.publisherAmerican Society for Microbiologyen_ZA
dc.rights© 2018, American Society for Microbiology. All Rights Reserved.en_ZA
dc.subjectX-ray crystallographyen_ZA
dc.subjectStructural characterizationen_ZA
dc.subjectSingle-point mutationen_ZA
dc.subjectSalt concentrationen_ZA
dc.subjectMetagenomicsen_ZA
dc.subjectDirected evolutionen_ZA
dc.subjectCatalytic efficienciesen_ZA
dc.subjectBiosynthesisen_ZA
dc.subjectCarbohydratesen_ZA
dc.subjectCatalyst activityen_ZA
dc.subjectCloningen_ZA
dc.subjectCrystal structureen_ZA
dc.subjectEfficiencyen_ZA
dc.subjectEnzymesen_ZA
dc.subjectEstersen_ZA
dc.subjectGenesen_ZA
dc.subjectSodium compoundsen_ZA
dc.subjectThermodynamic stabilityen_ZA
dc.subjectAcetyl xylan esterase (AcXE)en_ZA
dc.subjectCarbohydrate esterase 7 (CE7)en_ZA
dc.subjectSequence-based metagenomicsen_ZA
dc.subjectThermal stabilityen_ZA
dc.titleStructural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 familyen_ZA
dc.typePostprint Articleen_ZA

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