Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family
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Date
Authors
Adesioye, Fiyinfoluwa Adenike
Makhalanyane, Thulani Peter
Vikram, Surendra
Sewell, Bryan T.
Schubert, Wolf-Dieter
Cowan, Don A.
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Abstract
Please read abstract in the article.
Description
Keywords
X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability
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Citation
Adesioye FA, Makhalanyane TP, Vikram S, Sewell BT, Schubert W-D, Cowan DA. 2018. Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family. Appl Environ Microbiol 84:e02695-17. https://doi.org/10.1128/AEM.02695-17.