Extractives from Artemisia afra with anti-bacterial and anti-fungal properties
dc.contributor.author | Molokoane, Tumelo L. | |
dc.contributor.author | Kemboi, Douglas | |
dc.contributor.author | Siwe-Noundou, Xavier | |
dc.contributor.author | Famuyide, Ibukun Michael | |
dc.contributor.author | McGaw, Lyndy Joy | |
dc.contributor.author | Tembu, Vuyelwa J. | |
dc.contributor.email | lyndy.mcgaw@up.ac.za | en_US |
dc.date.accessioned | 2024-09-13T12:50:15Z | |
dc.date.available | 2024-09-13T12:50:15Z | |
dc.date.issued | 2023-10 | |
dc.description | SUPPLEMENTARY MATERIAL : TABLE S1: 1H ( H; J, Hz) NMR (400 MHZ) data for compounds A–H in CDCl3, MeOD and DMSO; TANLE S2: 13C ( c) (100.6 MHz) NMR data for compounds A–H in CDCl3, MeOD and DMSO and S2, FIGURES SA.1–SH.68: Spectral data for compounds (A–H). | en_US |
dc.description.abstract | Secondary metabolites were isolated using chromatographic techniques after being extracted sequentially from the roots of Artemisia afra using organic solvents such as ethanol, ethyl acetate, dichloromethane, and n-hexane. The isolated compounds were evaluated for anti-fungal, anti-bacterial, and cytotoxicity activities. Spectroscopic techniques, including Nuclear Magnetic Resonance (NMR), Fourier transform infrared (FTIR), and liquid chromatography–mass spectrometry (LC-MS), were used to elucidate the structures of the isolated compounds. The phytochemical investigation of A. afra led to the isolation of eight (A–H) compounds which were identified as 3b-taraxerol (A), 3b-taraxerol acetate (B), dodecyl-p-coumarate (C), ferulic acid (D), scopoletin (E), sitosterol-3-O-b-D-glucopyranoside (F), 3,5-di-O-feruloylquinic acid (G) and Isofraxidin-7-O-b-Dglucopyranoside (H) based on spectroscopic data. Compounds A, B, C, F, G, and H are known but were isolated for the first time from the roots of A. afra. The isolated compounds and extracts from A. afra exhibited good anti-fungal and anti-bacterial activity with dichloromethane and ethyl acetate crude extracts (0.078 mg/mL) and compound E (62.5 g/mL) showed good activities against Escherichia coli. Compounds C and F also showed good activity against Enterococcus faecalis with minimum inhibitory concentration (MIC) values of 62.5 and 31.25 g/mL, respectively. Extracts and compounds (A–H) exhibited anti-fungal and anti-bacterial properties and showed no toxicity when tested on Vero monkey kidney (Vero) cells. | en_US |
dc.description.department | Paraclinical Sciences | en_US |
dc.description.librarian | am2024 | en_US |
dc.description.sdg | SDG-15:Life on land | en_US |
dc.description.sponsorship | The National Research Foundation Research South Africa and Tshwane University of Technology (VJT). | en_US |
dc.description.uri | https://www.mdpi.com/journal/plants | en_US |
dc.identifier.citation | Molokoane, T.L.; Kemboi, D.; Siwe-Noundou, X.; Famuyide, I.M.; McGaw, L.J.; Tembu, V.J. Extractives from Artemisia afra with Anti-Bacterial and Anti-Fungal Properties. Plants 2023, 12, 3369. https://DOI.org/10.3390/plants12193369. | en_US |
dc.identifier.issn | 2223-7747 (online) | |
dc.identifier.other | 10.3390/plants12193369 | |
dc.identifier.uri | http://hdl.handle.net/2263/98203 | |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_US |
dc.subject | Artemisia afra | en_US |
dc.subject | Extractives | en_US |
dc.subject | Anti-bacterial | en_US |
dc.subject | Anti-fungal | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Nuclear magnetic resonance (NMR) | en_US |
dc.subject | Liquid chromatography–mass spectrometry (LC-MS) | en_US |
dc.subject | Fourier transform infrared (FTIR) | en_US |
dc.subject | SDG-15: Life on land | en_US |
dc.title | Extractives from Artemisia afra with anti-bacterial and anti-fungal properties | en_US |
dc.type | Article | en_US |
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