Extractives from Artemisia afra with anti-bacterial and anti-fungal properties

dc.contributor.authorMolokoane, Tumelo L.
dc.contributor.authorKemboi, Douglas
dc.contributor.authorSiwe-Noundou, Xavier
dc.contributor.authorFamuyide, Ibukun Michael
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorTembu, Vuyelwa J.
dc.contributor.emaillyndy.mcgaw@up.ac.zaen_US
dc.date.accessioned2024-09-13T12:50:15Z
dc.date.available2024-09-13T12:50:15Z
dc.date.issued2023-10
dc.descriptionSUPPLEMENTARY MATERIAL : TABLE S1: 1H ( H; J, Hz) NMR (400 MHZ) data for compounds A–H in CDCl3, MeOD and DMSO; TANLE S2: 13C ( c) (100.6 MHz) NMR data for compounds A–H in CDCl3, MeOD and DMSO and S2, FIGURES SA.1–SH.68: Spectral data for compounds (A–H).en_US
dc.description.abstractSecondary metabolites were isolated using chromatographic techniques after being extracted sequentially from the roots of Artemisia afra using organic solvents such as ethanol, ethyl acetate, dichloromethane, and n-hexane. The isolated compounds were evaluated for anti-fungal, anti-bacterial, and cytotoxicity activities. Spectroscopic techniques, including Nuclear Magnetic Resonance (NMR), Fourier transform infrared (FTIR), and liquid chromatography–mass spectrometry (LC-MS), were used to elucidate the structures of the isolated compounds. The phytochemical investigation of A. afra led to the isolation of eight (A–H) compounds which were identified as 3b-taraxerol (A), 3b-taraxerol acetate (B), dodecyl-p-coumarate (C), ferulic acid (D), scopoletin (E), sitosterol-3-O-b-D-glucopyranoside (F), 3,5-di-O-feruloylquinic acid (G) and Isofraxidin-7-O-b-Dglucopyranoside (H) based on spectroscopic data. Compounds A, B, C, F, G, and H are known but were isolated for the first time from the roots of A. afra. The isolated compounds and extracts from A. afra exhibited good anti-fungal and anti-bacterial activity with dichloromethane and ethyl acetate crude extracts (0.078 mg/mL) and compound E (62.5 g/mL) showed good activities against Escherichia coli. Compounds C and F also showed good activity against Enterococcus faecalis with minimum inhibitory concentration (MIC) values of 62.5 and 31.25 g/mL, respectively. Extracts and compounds (A–H) exhibited anti-fungal and anti-bacterial properties and showed no toxicity when tested on Vero monkey kidney (Vero) cells.en_US
dc.description.departmentParaclinical Sciencesen_US
dc.description.librarianam2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipThe National Research Foundation Research South Africa and Tshwane University of Technology (VJT).en_US
dc.description.urihttps://www.mdpi.com/journal/plantsen_US
dc.identifier.citationMolokoane, T.L.; Kemboi, D.; Siwe-Noundou, X.; Famuyide, I.M.; McGaw, L.J.; Tembu, V.J. Extractives from Artemisia afra with Anti-Bacterial and Anti-Fungal Properties. Plants 2023, 12, 3369. https://DOI.org/10.3390/plants12193369.en_US
dc.identifier.issn2223-7747 (online)
dc.identifier.other10.3390/plants12193369
dc.identifier.urihttp://hdl.handle.net/2263/98203
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectArtemisia afraen_US
dc.subjectExtractivesen_US
dc.subjectAnti-bacterialen_US
dc.subjectAnti-fungalen_US
dc.subjectCytotoxicityen_US
dc.subjectNuclear magnetic resonance (NMR)en_US
dc.subjectLiquid chromatography–mass spectrometry (LC-MS)en_US
dc.subjectFourier transform infrared (FTIR)en_US
dc.subjectSDG-15: Life on landen_US
dc.titleExtractives from Artemisia afra with anti-bacterial and anti-fungal propertiesen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Molokoane_Extractives_2023.pdf
Size:
781.83 KB
Format:
Adobe Portable Document Format
Description:
Article
Loading...
Thumbnail Image
Name:
Molokoane_ExtractivesSuppl_2023.pdf
Size:
4.65 MB
Format:
Adobe Portable Document Format
Description:
Supplementary Material

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: