Extractives from Artemisia afra with anti-bacterial and anti-fungal properties
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Date
Authors
Molokoane, Tumelo L.
Kemboi, Douglas
Siwe-Noundou, Xavier
Famuyide, Ibukun Michael
McGaw, Lyndy Joy
Tembu, Vuyelwa J.
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Secondary metabolites were isolated using chromatographic techniques after being extracted
sequentially from the roots of Artemisia afra using organic solvents such as ethanol, ethyl
acetate, dichloromethane, and n-hexane. The isolated compounds were evaluated for anti-fungal,
anti-bacterial, and cytotoxicity activities. Spectroscopic techniques, including Nuclear Magnetic
Resonance (NMR), Fourier transform infrared (FTIR), and liquid chromatography–mass spectrometry
(LC-MS), were used to elucidate the structures of the isolated compounds. The phytochemical
investigation of A. afra led to the isolation of eight (A–H) compounds which were identified as
3b-taraxerol (A), 3b-taraxerol acetate (B), dodecyl-p-coumarate (C), ferulic acid (D), scopoletin (E),
sitosterol-3-O-b-D-glucopyranoside (F), 3,5-di-O-feruloylquinic acid (G) and Isofraxidin-7-O-b-Dglucopyranoside
(H) based on spectroscopic data. Compounds A, B, C, F, G, and H are known
but were isolated for the first time from the roots of A. afra. The isolated compounds and extracts
from A. afra exhibited good anti-fungal and anti-bacterial activity with dichloromethane and ethyl
acetate crude extracts (0.078 mg/mL) and compound E (62.5 g/mL) showed good activities against
Escherichia coli. Compounds C and F also showed good activity against Enterococcus faecalis with
minimum inhibitory concentration (MIC) values of 62.5 and 31.25 g/mL, respectively. Extracts and
compounds (A–H) exhibited anti-fungal and anti-bacterial properties and showed no toxicity when
tested on Vero monkey kidney (Vero) cells.
Description
SUPPLEMENTARY MATERIAL : TABLE S1: 1H ( H; J, Hz) NMR (400 MHZ) data for compounds A–H in CDCl3, MeOD and DMSO; TANLE S2: 13C ( c) (100.6 MHz) NMR data for compounds A–H in CDCl3, MeOD and DMSO and S2, FIGURES SA.1–SH.68: Spectral data for compounds (A–H).
Keywords
Artemisia afra, Extractives, Anti-bacterial, Anti-fungal, Cytotoxicity, Nuclear magnetic resonance (NMR), Liquid chromatography–mass spectrometry (LC-MS), Fourier transform infrared (FTIR), SDG-15: Life on land
Sustainable Development Goals
SDG-15:Life on land
Citation
Molokoane, T.L.; Kemboi,
D.; Siwe-Noundou, X.; Famuyide,
I.M.; McGaw, L.J.; Tembu, V.J.
Extractives from Artemisia afra with
Anti-Bacterial and Anti-Fungal
Properties. Plants 2023, 12, 3369.
https://DOI.org/10.3390/plants12193369.