Abstract:
Oxidase and peroxidase enzymes have attracted attention in various biotechnological
industries due to their ease of synthesis, wide range of applications, and operation under mild
conditions. Their applicability, however, is limited by their poor stability in harsher conditions
and their non-reusability. As a result, several approaches such as enzyme engineering, medium
engineering, and enzyme immobilization have been used to improve the enzyme properties. Several
materials have been used as supports for these enzymes to increase their stability and reusability.
This review focusses on the immobilization of oxidase and peroxidase enzymes on metal and metal
oxide nanoparticle-polymer composite supports and the different methods used to achieve the
immobilization. The application of the enzyme-metal/metal oxide-polymer biocatalysts in biosensing
of hydrogen peroxide, glucose, pesticides, and herbicides as well as blood components such as
cholesterol, urea, dopamine, and xanthine have been extensively reviewed. The application of the
biocatalysts in wastewater treatment through degradation of dyes, pesticides, and other organic
compounds has also been discussed.
