dc.contributor.author |
Sandhu, Armaan Kaur
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|
dc.contributor.author |
Brown, McKenzie Rae
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|
dc.contributor.author |
Subramanian, Senthil
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dc.contributor.author |
Brozel, Volker S.
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dc.date.accessioned |
2024-06-18T04:54:56Z |
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dc.date.available |
2024-06-18T04:54:56Z |
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dc.date.issued |
2023-05-18 |
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dc.description |
DATA AVAILABILITY STATEMENT : The raw data supporting the conclusions of this article will be
made available by the authors, without undue reservation. |
en_US |
dc.description.abstract |
INTRODUCTION : Bradyrhizobium diazoe ciens, a symbiotic nitrogen fixer for soybean, forms nodules after developing a symbiotic association with the root. For this association, bacteria need to move toward and attach to the root. These steps are mediated by the surface and phenotypic cell properties of bacteria and secreted root exudate compounds. Immense work has been carried out on nodule formation and nitrogen fixation, but little is known about the phenotype of these microorganisms under the influence of differrent root exudate chemical compounds (RECCs) or how this phenotype impacts the root attachment ability. METHODS : To address this knowledge gap, we studied the impact of 12 different RECCs, one commonly used carbon source, and soil-extracted solubilized organic matter (SESOM) on attachment and attachment-related properties of B. diazoe ciens USDA110. We measured motility-related properties (swimming, swarming, chemotaxis, and flagellar expression), attachment-related properties (surface hydrophobicity, biofilm formation, and attachment to cellulose and soybean roots), and surface polysaccharide properties (colony morphology, exopolysaccharide quantification, lectin binding profile, and lipopolysaccharide profiling). RESULTS AND DISCUSSION : We found that USDA 110 displays a high degree of surface phenotypic plasticity when grown on the various individual RECCs. Some of the RECCs played specific roles in modulating the motility and root attachment processes. Serine increased cell surface hydrophobicity and root and cellulose attachment, with no EPS formed. Gluconate and lactate increased EPS production and biofilm formation, while decreasing hydrophobicity and root attachment, and ra nose and gentisate promoted motility and chemotaxis. The results also indicated that the biofilm formation trait on hydrophilic surfaces (polystyrene) cannot be related to the attachment ability of Bradyrhizobiumto the soybean root. Among the tested phenotypic properties, bacterial cell surface hydrophobicity was the one with a significant impact on root attachment ability. We conclude that USDA 110 displays surface plasticity properties and attachment phenotype determined by individual RECCs from the soybean. Conclusions made based on its behavior in standard carbon sources, such as arabinose or mannitol, do not hold for its behavior in soil. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.librarian |
am2024 |
en_US |
dc.description.sdg |
SDG-15:Life on land |
en_US |
dc.description.sponsorship |
A National Science Foundation Assistantship, the National Science Foundation/EPSCoR RII Track-1: Building on The 2020 Vision: Expanding Research, Education, and Innovation in South Dakota, and by the South Dakota Board of Regents. |
en_US |
dc.description.uri |
http://www.frontiersin.org/Microbiology |
en_US |
dc.identifier.citation |
Sandhu, A.K., Brown, M.R., Subramanian, S. & Brozel, V.S. (2023) Bradyrhizobium diazoe ciens USDA 110 displays plasticity in the attachment phenotype when grown in different soybean root exudate compounds. Frontiers in Microbiology 14:1190396.
DOI: 10.3389/fmicb.2023.1190396. |
en_US |
dc.identifier.issn |
1664-302X (online) |
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dc.identifier.other |
10.3389/fmicb.2023.1190396 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/96505 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Frontiers Media |
en_US |
dc.rights |
© 2023 Sandhu, Brown, Subramanian and
Brözel. This is an open-access article
distributed under the terms of the Creative
Commons Attribution License (CC BY). |
en_US |
dc.subject |
Bradyrhizobium diazoefficiens |
en_US |
dc.subject |
Soybean |
en_US |
dc.subject |
Root |
en_US |
dc.subject |
Attachment |
en_US |
dc.subject |
Biofilm |
en_US |
dc.subject |
Phenotype |
en_US |
dc.subject |
Root exudate |
en_US |
dc.subject |
Phenotypic plasticity |
en_US |
dc.subject |
Bradyrhizobium diazoe ciens |
en_US |
dc.subject |
Root exudate chemical compound (RECC) |
en_US |
dc.subject |
Soil-extracted solubilized organic matter (SESOM) |
en_US |
dc.subject |
SDG-15: Life on land |
en_US |
dc.title |
Bradyrhizobium diazoefficiens USDA 110 displays plasticity in the attachment phenotype when grown in different soybean root exudate compounds |
en_US |
dc.type |
Article |
en_US |