dc.contributor.author |
Labuschagne, Christiaan
|
|
dc.contributor.author |
Karzis, Joanne
|
|
dc.contributor.author |
Britz, Hans
|
|
dc.contributor.author |
Petzer, Inge-Marie
|
|
dc.date.accessioned |
2023-04-24T09:02:44Z |
|
dc.date.available |
2023-04-24T09:02:44Z |
|
dc.date.issued |
2022-11-12 |
|
dc.description |
Figure S1: Gel images showing 1% agarose
gel of MLST PCR products: (M) Marker, NEB Fast ladder (N3238S), (Ne) Template Negative control, of
each of the seven housekeeping genes (PyrR, glPk, Gmk, AdhP, Xpt, PTA, InfB) for the first 5 S. simulans
isolates from water buffalo. Table S1: Raw data of MALDI-TOF identification of Staphylococcus
simulans isolated from milk samples of water buffalo and dairy cows, which was performed in
duplicate. Data S1: The FASTA format, which is the concatenated (combined) MLST sequencing data.
Image S1: The alignment image of concatenated (combined) MLST sequencing data. |
en_US |
dc.description |
SUPPLEMENTARY MATERIAL : FIGURE S1 : Gel images showing 1% agarose gel of MLST PCR products: (M) Marker, NEB Fast ladder (N3238S), (Ne) Template Negative control, of each of the seven housekeeping genes (PyrR, glPk, Gmk, AdhP, Xpt, PTA, InfB) for the first 5 S. simulans isolates from water buffalo. TABLE S1 : Raw data of MALDI-TOF identification of Staphylococcus simulans isolated from milk samples of water buffalo and dairy cows, which was performed in duplicate. DATA S1 : The FASTA format, which is the concatenated (combined) MLST sequencing data. IMAGE S1 : The alignment image of concatenated (combined) MLST sequencing data. |
en_US |
dc.description.abstract |
Water buffalo produce a tenth of milk for global human consumption. Non-aureus staphylococci
(NAS) are among the most commonly isolated bacteria from mastitis in water buffalo and
dairy cows. These results described the initial characterisation of 17 NAS—15 Staphylococcus simulans
and two Staphylococcus chromogenes from a water buffalo herd (n = 44) in South Africa. The isolates
were identified by classical microbiology, MALDI-TOF, and 16S rRNA, and the disc diffusion method
determined the antibiotic susceptibility. A multi-locus sequence typing scheme (MLST) was developed
to determine S. simulans sequence types (ST), by defining and comparing seven housekeeping
gene fragment sequences. Sequence typing confirmed all 15 S. simulans isolates from water buffalo
which belonged to a single ST, genetically distant from the six bovine STs isolated from adjacent
farms, which also varied, indicating no current bacterial transfer between species. The antibiotic
resistance patterns of S. simulans varied between beta-lactams. The mean milk somatic cell count
(SCC) for the water buffalo milk samples was 166,500 cells/mL milk. This information offers insights
into the epidemiology and comparison among isolates from various origins, which leads to effective
proactive mastitis strategies resulting in safe, high-quality dairy products from water buffalo and
dairy cows for human consumption. |
en_US |
dc.description.department |
Production Animal Studies |
en_US |
dc.description.librarian |
am2023 |
en_US |
dc.description.sponsorship |
The National Research Foundation (NRF) of South Africa. |
en_US |
dc.description.uri |
https://www.mdpi.com/journal/antibiotics |
en_US |
dc.identifier.citation |
Labuschagne, C.; Karzis, J.;
Britz, H.; Petzer, I.-M. Characterisation
of Staphylococci Isolated from Milk
Samples of aWater Buffalo Herd.
Antibiotics 2022, 11, 1609. https://DOI.org/10.3390/antibiotics11111609. |
en_US |
dc.identifier.issn |
2079-6382 |
|
dc.identifier.other |
10.3390/antibiotics11111609 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/90434 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
MDPI |
en_US |
dc.rights |
© 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license. |
en_US |
dc.subject |
Staphylococcus simulans |
en_US |
dc.subject |
Water buffalo |
en_US |
dc.subject |
Multi-locus sequence typing (MLST) |
en_US |
dc.subject |
Mastitis |
en_US |
dc.subject |
Antibiotic resistance |
en_US |
dc.subject |
Non-aureus staphylococci (NAS) |
en_US |
dc.subject |
Water buffalo (Bubalus bubalis) |
en_US |
dc.title |
Characterisation of staphylococci isolated from milk samples of a water buffalo herd |
en_US |
dc.type |
Article |
en_US |