Abstract:
Amidst an ever-evolving pandemic, the demand for timely and accurate diagnosis of
coronavirus disease 2019 (COVID-19) continues to increase. Critically, managing and
containing the spread of the disease requires expedient testing of infected individuals.
Presently, the gold standard for the diagnosis of severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) infection remains the polymerase chain reaction (PCR) test. Potential
vulnerabilities of this testing methodology can range from preanalytical variables to laboratoryrelated
analytical factors and, ultimately, to the interpretation of results.
