Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum
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Date
Authors
Barnes, Irene
Cortinas, Maria-Noel
Wingfield, Michael J.
Wingfield, Brenda D.
Journal Title
Journal ISSN
Volume Title
Publisher
Blackwell
Abstract
Twelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 primer pairs designed, 12 were polymorphic. These markers, screened on two populations representing 42 isolates, produced 40 alleles across all loci with an allelic diversity of 0.09–0.76 per locus. Cross-species amplification showed variable success with Dothistroma rhabdoclinis and Mycosphaerella dearnessi and some sequence variation within isolates of Dothistroma pini. These markers will be used to further study the population structure and diversity of D. septosporum.
Description
Keywords
Cross-species amplification, Fungi, Haploid, Mycosphaerella pini, Population genetics, Sequence variation
Sustainable Development Goals
Citation
Barnes, I, Cortinas MN, Wingfield, MJ & Wingfield, BD 2008, ‘Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum’, Molecular Ecology Resources, vol. 8, no. 5, pp. 1026-1029.[http://www.blackwellpublishing.com]