Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum

dc.contributor.authorBarnes, Irene
dc.contributor.authorCortinas, Maria-Noel
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorWingfield, Brenda D.
dc.date.accessioned2009-02-13T06:32:53Z
dc.date.available2009-02-13T06:32:53Z
dc.date.issued2008
dc.description.abstractTwelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 primer pairs designed, 12 were polymorphic. These markers, screened on two populations representing 42 isolates, produced 40 alleles across all loci with an allelic diversity of 0.09–0.76 per locus. Cross-species amplification showed variable success with Dothistroma rhabdoclinis and Mycosphaerella dearnessi and some sequence variation within isolates of Dothistroma pini. These markers will be used to further study the population structure and diversity of D. septosporum.en_US
dc.identifier.citationBarnes, I, Cortinas MN, Wingfield, MJ & Wingfield, BD 2008, ‘Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum’, Molecular Ecology Resources, vol. 8, no. 5, pp. 1026-1029.[http://www.blackwellpublishing.com]en_US
dc.identifier.issn1755-0998
dc.identifier.other10.1111/j.1755-0998.2008.02142.x
dc.identifier.urihttp://hdl.handle.net/2263/8894
dc.language.isoenen_US
dc.publisherBlackwellen_US
dc.rightsBlackwell. The definitive version of the article is available at www.blackwell-synergy.com. This article is embargoed by the publisher until June 2009.en_US
dc.subjectCross-species amplificationen_US
dc.subjectFungien_US
dc.subjectHaploiden_US
dc.subjectMycosphaerella pinien_US
dc.subjectPopulation geneticsen_US
dc.subjectSequence variationen_US
dc.subject.lcshFungal populations -- Research
dc.subject.lcshPolymerase chain reaction
dc.titleMicrosatellite markers for the red band needle blight pathogen, Dothistroma septosporumen_US
dc.typePostprint Articleen_US

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