DATA AVAILABILITY STATEMENT : All FastQ sequence
files generated from this work are available from
the National Center for Biotechnology Information’s
Short Reads Archive, under BioProject number
PRJNA777568, Accession numbers SRI168760
-SRI168784. All relevant data are within the
manuscript and its Supporting Information files.
SUPPLEMENTARY MATERIAL : S1 Fig.
A: Alpha diversity box-plots showing Chao1 richness estimates per sample group. *Significant at P < 0,05. B: Alpha diversity box-plots showing Shannon diversity estimates per sample group. *Significant at P < 0,05. C: Alpha diversity box-plots showing Simpson’s diversity estimates per sample group. *Significant at P < 0,05.
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S2 Fig. UpSetR intersection plot showing number of unique and shared taxa at family level between faeces, milk and blood groups.
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S1 Table. Read counts tracked through the DADA2 pipeline including ASV counts, richness and genus level-resolved ASVs per sample.
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S2 Table. Alpha diversity values calculated using Shannon, Simpson and Chao1 indices.
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S3 Table. Total number of taxa detected per taxonomic rank across bovine faeces, milk and blood.
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S4 Table. Top 15 abundant taxa with their respective overall rankings and distribution across the three sample groups.
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S5 Table. Prevalence of potentially pathogenic genera of veterinary significance per sample group.
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S6 Table. Bacterial taxa shared between bovine faeces, milk and blood and their overall raw and relative abundances.
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S7 Table. Genus-level taxa exclusively detected and shared between faeces, milk and blood samples.
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S8 Table.
A-C Differentially abundant taxa between blood and faeces; blood and milk and; faeces and milk (Padj < 0,01).
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S1 Raw images. Gel electrophoresis image of Anaplasma PCR targeting the 16S rRNA gene from blood samples.
Image taken under UV transillumination using Enduro™ GOS gel documentation system. Lane 1 = 1 kb DNA ladder; 2–10 = Anaplasma positive samples; 11 = nuclease free H20 (-ve); 12 = A. marginale (+ve).
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