dc.contributor.author |
Mafuyeka, Rendani
|
|
dc.contributor.author |
Webber, Lynne M.
|
|
dc.contributor.author |
Becker, Piet J.
|
|
dc.contributor.author |
Mayaphi, Simnikiwe Horatious
|
|
dc.date.accessioned |
2022-10-11T12:58:54Z |
|
dc.date.available |
2022-10-11T12:58:54Z |
|
dc.date.issued |
2021-03-12 |
|
dc.description.abstract |
BACKGROUND : The human immunodeficiency virus type-2 (HIV-2) prevalence in South Africa
(SA) is unknown, however, sporadic cases have been reported. Human immunodeficiency
virus -1 and 2 differentiation is not part of most South African public laboratories’ testing
algorithm. Human immunodeficiency virus -2 diagnosis using serology assays may be
complicated by HIV-1 and HIV-2 antibody cross-reactivity.
OBJECTIVES : To determine the proportion of HIV-2 infections in specimens that tested HIV-1/2
positive at a public laboratory in Tshwane.
METHOD: A total of 480 specimens that were previously tested with fourth generation ELISA
platforms (Modular E170 [Roche, Switzerland] and Architect i2000 [Abbott, Germany]) were
randomly selected. Human immunodeficiency virus -1 and 2 antibody differentiation testing
was carried out using the Multispot HIV-1/2 rapid assay (Bio-Rad Laboratories, USA). An inhouse nested HIV-2 PCR assay targeting the 5'-long terminal repeats (5'-LTR) region was
evaluated and used as a confirmatory test.
RESULT: The study tested 480 HIV-1/2 seropositive patients and their mean age was 36.7 years
(range 3–82 years). Of the 480 patients, 292 (60.8%) were female, 182 (37.9%) were male and
6 (1.3%) were not specified. Human immunodeficiency virus differentiation results were as
follows: 466 (97.1%) were positive for only HIV-1 antibodies, 11 (2.3%) [95%CI: (0.98%; 3.74%)]
were positive for both HIV-1 and HIV-2 antibodies, 3 (0.6%) were negative for both antibodies
and none were positive for only HIV-2 antibodies. Of the 11 specimens with both HIV-1 and
HIV-2 antibodies, seven had sufficient volume for confirmatory testing and were all negative
on the in-house HIV-2 PCR assay.
CONCLUSION: The multispot HIV-1/2 rapid assay demonstrated cross-reactivity
between HIV-1 and HIV-2 antibodies. Human immunodeficiency virus -2 infections were
not detected. |
en_US |
dc.description.department |
Medical Virology |
en_US |
dc.description.librarian |
dm2022 |
en_US |
dc.description.sponsorship |
NHLS Research Trust; Discovery Foundation and University of Pretoria Research grant. |
en_US |
dc.description.uri |
http://www.sajhivmed.org.za/index.php/hivmed |
en_US |
dc.identifier.citation |
Mafuyeka, R.T., Webber, L.M., Becker, P. & Mayaphi, S.H. HIV-1/2 differentiation in a South African public laboratory. Southern African Journal of HIV Medicine 2021;22(1), a1185. https://doi.org/10.4102/sajhivmed.v22i1.1185. |
en_US |
dc.identifier.issn |
1608-9693 (print) |
|
dc.identifier.issn |
2078-6751 (online) |
|
dc.identifier.other |
10.4102/ sajhivmed.v22i1.1185 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/87636 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
AOSIS |
en_US |
dc.rights |
© 2021. The Authors.
Licensee: AOSIS. This work
is licensed under the
Creative Commons
Attribution License. |
en_US |
dc.subject |
HIV-1/2 differentiation |
en_US |
dc.subject |
HIV-2 testing |
en_US |
dc.subject |
HIV1/2 antibody cross reaction |
en_US |
dc.subject |
HIV-2 in South Africa |
en_US |
dc.subject |
HIV-2 PCR |
en_US |
dc.subject |
Human immunodeficiency virus type-2 (HIV-2) |
en_US |
dc.subject |
South Africa (SA) |
en_US |
dc.subject |
Polymerase chain reaction (PCR) |
en_US |
dc.title |
HIV-1/2 differentiation in a South African public laboratory |
en_US |
dc.type |
Article |
en_US |