Abstract:
Purpose: During a pathological inflammation, macrophages are activated to produce accumulation
of inflammatory mediators such as induced-cyclooxygenase-2 (COX-2), 15-
lipoxygenase (15-LOX) and pro-inflammatory cytokines. Pathological inflammation is
a significant problem in many chronic diseases. As a result, more research into natural
remedies with anti-inflammatory potential is crucial. Since ancient times, psilocybincontaining
mushrooms, also known as magic mushrooms, were used for mind healing and
also to advance the quality of life. However, not much is known about their antiinflammatory
potential. This study aimed at investigating the anti-inflammatory effects of
four psilocybin-containing mushrooms (Panaeolus cyanescens, Psilocybe natalensis,
Psilocybe cubensis and Psilocybe cubensis leucistic A+ strain) from genus Panaeolus and
Psilocybe for the first time in vitro on 15-LOX activity and also on lipopolysaccharide
(LPS)-induced inflammation in human U937 macrophage cells.
Methods: Mushrooms were grown and extracted with boiling hot water. Effects of the four
water extracts on 15-LOX activity were determined. Confluent human U937 cells were
differentiated with phorbol 12-myristate 13-acetate and treated with the hot-water extracts (25
and 50 μg/mL) 2 hours before being stimulated with 1 μg/mL LPS over 24 hours. Quercetin was
used as a positive control. Control cells were differentiated but not LPS-induced nor treated.
Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 concentrations were
measured. Levels of COX-2 and mitochondrial activity were also determined.
Results: The four water extracts had poor 15-LOX inhibition activity with IC50 > 250 μg/mL.
Extracts were safe at the concentration studied and inhibited the LPS-induced production of proinflammatory
mediators, TNF-α and IL-1β significantly and lowered IL-6 and COX-2 concentrations in treated human U937 macrophage cells. Water extracts also increased percentage viability
of treated cells and levels of anti-inflammatory IL-10 non-significantly.
Conclusion: The study suggested that the hot-water extracts of the four psilocybincontaining
magic mushrooms have potential anti-inflammatory effects executed by downregulating
pro-inflammatory mediators.
