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dc.contributor.author | Eloff, Jacobus Nicolaas![]() |
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dc.date.accessioned | 2022-07-22T13:04:08Z | |
dc.date.available | 2022-07-22T13:04:08Z | |
dc.date.issued | 2021-08 | |
dc.description.abstract | Many scientists investigate the potential of finding new antibiotics from plants, leading to more than a thousand publications per year. Many different minimum inhibitory concentrations of extracts have been proposed to decide if an extract has interesting activity that could lead to the discovery of a new antibiotic. To date, no rational explanation has been given for the selection criteria different authors have used. The cumulative percentage of plant extracts with different activities from a large experiment determining the activity of 714 acetone tree leaf extracts of 537 different South African tree species against 4 nosocomial pathogenic bacteria and 2 yeasts was calculated using a widely accepted serial dilution microplate method with p-iodonitrotetrazolium violet as indicator of growth. All the extracts were active at a concentration of 2.5 mg/mL. The formula, % of active extracts = 439 × minimum inhibitory concentration in mg/mL1.5385, describes the results for minimum inhibitory concentrations below 0.16 mg/mL, with a correlation coefficient of 0.9998. A rational approach could be to determine the minimum inhibitory concentrations of the most active 1, 3, 9, 25, 50, and > 50% of a large number of plant extracts investigated against these six important microbial pathogens. Starting with an extract concentration of 10 mg/mL, I propose the following classification based on minimum inhibitory concentrations: outstanding activity < 0.02 mg/mL, excellent activity 0.021 – 0.04 mg/mL, very good activity 041 – 0.08 mg/mL, good activity 0.081 – 0.16 mg/mL, average activity 0.161 – 0.32 mg/mL, and weak activity > 0.32 mg/mL. Higher minimum inhibitory concentrations may still be effective in ethnopharmacological studies.Many scientists investigate the potential of finding new antibiotics from plants, leading to more than a thousand publications per year. Many different minimum inhibitory concentrations of extracts have been proposed to decide if an extract has interesting activity that could lead to the discovery of a new antibiotic. To date, no rational explanation has been given for the selection criteria different authors have used. The cumulative percentage of plant extracts with different activities from a large experiment determining the activity of 714 acetone tree leaf extracts of 537 different South African tree species against 4 nosocomial pathogenic bacteria and 2 yeasts was calculated using a widely accepted serial dilution microplate method with p-iodonitrotetrazolium violet as indicator of growth. All the extracts were active at a concentration of 2.5 mg/mL. The formula, % of active extracts = 439 × minimum inhibitory concentration in mg/mL1.5385, describes the results for minimum inhibitory concentrations below 0.16 mg/mL, with a correlation coefficient of 0.9998. A rational approach could be to determine the minimum inhibitory concentrations of the most active 1, 3, 9, 25, 50, and > 50% of a large number of plant extracts investigated against these six important microbial pathogens. Starting with an extract concentration of 10 mg/mL, I propose the following classification based on minimum inhibitory concentrations: outstanding activity < 0.02 mg/mL, excellent activity 0.021 – 0.04 mg/mL, very good activity 041 – 0.08 mg/mL, good activity 0.081 – 0.16 mg/mL, average activity 0.161 – 0.32 mg/mL, and weak activity > 0.32 mg/mL. Higher minimum inhibitory concentrations may still be effective in ethnopharmacological studies. | en_US |
dc.description.department | Paraclinical Sciences | en_US |
dc.description.librarian | hj2022 | en_US |
dc.description.sponsorship | The National Research Foundation, South Africa Incentive funding. | en_US |
dc.description.uri | https://www.thieme-connect.com/products/ejournals/journal/10.1055/s-00000058 | en_US |
dc.identifier.citation | Eloff, J.N. 2021, 'A proposal towards a rational classification of the antimicrobial activity of acetone tree leaf extracts in a search for new antimicrobials', Planta Medica, vol. 87, no. 10/11, pp. 836-840, DOI: 10.1055/a-1482-1410. | en_US |
dc.identifier.issn | 0032-0943 (print) | |
dc.identifier.issn | 1439-0221 (online) | |
dc.identifier.other | 10.1055/a-1482-1410 | |
dc.identifier.uri | https://repository.up.ac.za/handle/2263/86408 | |
dc.language.iso | en | en_US |
dc.publisher | Georg Thieme Verlag | en_US |
dc.rights | © 2021. Thieme. All rights reserved. | en_US |
dc.subject | Antimicrobial | en_US |
dc.subject | Minimum inhibitory concentration (MIC) | en_US |
dc.subject | Tree leaves | en_US |
dc.title | A proposal towards a rational classification of the antimicrobial activity of acetone tree leaf extracts in a search for new antimicrobials | en_US |
dc.type | Postprint Article | en_US |