dc.contributor.author |
Kabwe, Kapambwe Peter
|
|
dc.contributor.author |
Nsibande, Sifiso Albert
|
|
dc.contributor.author |
Lemmer, Yolandy
|
|
dc.contributor.author |
Pilcher, Lynne A.
|
|
dc.contributor.author |
Forbes, Patricia B.C.
|
|
dc.date.accessioned |
2022-04-13T09:40:12Z |
|
dc.date.available |
2022-04-13T09:40:12Z |
|
dc.date.issued |
2022-02 |
|
dc.description.abstract |
This work explores the potential use of cadmium-based quantum dots (QDs) coupled to mycolic acids (MAs) as a fluorescent probe to detect anti-MA antibodies which are biomarkers for tuberculosis (TB). The use of free MAs as antigens for the serodiagnosis of TB is known but has not been developed into a point of care test. This study focuses on the synthesis, solubility, and lateral flow of QDs coupled to MAs. Water-soluble CdSe/ZnS QDs capped with l-cysteine were synthesised and covalently coupled to MAs via amide linkages to form a water-soluble fluorescent probe: MA-CdSe/ZnS QDs. The MA-CdSe/ZnS QDs showed broad absorption bands and coupling, confirmed by the presence of amide bonds in the Fourier-transform infrared (FTIR) spectrum, resulting in a blue shift in fluorescence. Powder X-ray diffraction (XRD) revealed a shift and increase in the number of peaks for MA-CdSe/ZnS QDs relative to the L-cys-CdSe/ZnS QDs, suggesting that coupling changed the crystal structure. The average particle size of MA-CdSe/ZnS QDs was ~3.0 nm. Visual paper-based lateral flow of MA-CdSe/ZnS QDs was achieved on strips of nitrocellulose membrane with both water and membrane blocking solution eluents. The highly fluorescent MA-CdSe/ZnS QDs showed good water solubility and lateral flow, which are important properties for fluorescence sensing applications. |
en_US |
dc.description.department |
Chemistry |
en_US |
dc.description.embargo |
2022-11-23 |
en_US |
dc.description.librarian |
hj2021 |
en_US |
dc.description.sponsorship |
TWAS-NRF doctoral scholarship funding and a University of Pretoria postgraduate student bursary. |
en_US |
dc.description.uri |
http://wileyonlinelibrary.com/journal/bio |
en_US |
dc.identifier.citation |
Kabwe, K.P., Nsibande, S.A., Lemmer, Y. et al. 2022, 'Synthesis and characterisation of quantum dots coupled to mycolic acids as a water-soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis', Luminescence, vol. 37, no. 2, pp. 278-289, doi: 10.1002/bio.4170. |
en_US |
dc.identifier.issn |
1522-7235 (print) |
|
dc.identifier.issn |
1522-7243 (online) |
|
dc.identifier.other |
10.1002/bio.4170 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/84878 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.rights |
© 2021 John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article : 'Synthesis and characterisation of quantum dots coupled to mycolic acids as a water-soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis', Luminescence, vol. 37, no. 2, pp. 278-289, 2022, doi : 10.1002/bio.4170. The definite version is available at : http://wileyonlinelibrary.com/journal/bio. |
en_US |
dc.subject |
Quantum dots (QDs) |
en_US |
dc.subject |
Biomarkers |
en_US |
dc.subject |
Mycolic acids (MAs) |
en_US |
dc.subject |
Tuberculosis (TB) |
en_US |
dc.subject |
Fluorescence |
en_US |
dc.subject |
Fourier-transform infrared spectroscopy (FTIR) |
en_US |
dc.subject |
X-ray diffraction (XRD) |
en_US |
dc.title |
Synthesis and characterisation of quantum dots coupled to mycolic acids as a water-soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis |
en_US |
dc.type |
Postprint Article |
en_US |